摘要
目的利用分子生物学技术,研究腔阔盘吸虫的分类。方法提取腔阔盘吸虫基因组DNA,利用保守引物,PCR扩增18SrRNA片段并测序。应用DNAStar和MEGA3软件,分析腔阔盘吸虫与其他双腔吸虫18SrRNA的同源性,在此基础上绘制出系统进化树,确定它们的进化关系。结果腔阔盘吸虫和其他双腔吸虫18SrRNA的同源性很高,其中腔阔盘吸虫和支双腔吸虫(D.dendriticum)的差异最大,为2.42%,而与愁体吸虫(L.collurionis)的差异较小,为1.75%;D.dendriticum和分叶短腺吸虫(B.lobatum)的进化关系相对较近,差异为1.09%。结论双腔科吸虫的18SrRNA序列非常保守,腔阔盘吸虫和L.collurionis的亲缘关系最近,而与B.lobatum和D.dendriticum在进化上关系相对较远。
Objective To elucidate the taxonomic position of Eurytrema coelmaticum by using molecular technology. Methods 18S rRNA fragment was amplified from E. coelmaticum genomic DNA by specific conservative primers and sequenced. Homology and phylogenic tree of 18S rRNA sequences between E. coelmaticum and other Dicrocoeliidae trematodes were analyzed and constructed by DNAStar and MEGA3 respectively, and their evolutionary relationship was determined. Results E. coelmaticum 18S rRNA sequence was with high homology to those from Dicrocoelium dendriticum, Lyperosomum eollurionis and Brachylecithum lobatum. Among them, the diversity of E. coelmatioum from D. dendriticum was 2.42%, and that from L. collurionis was 1.75%; D. dendriticum and B. lobatum were closer in evolution only and and with 1.09% diversity. Conclusion the sequences are highly conservative B. lobatum. For Dicrocoeliidae trematodes, classification based on 18S rRNA target is valid E. coelmaticum is evolutionarily closer to L. collurionis than to D. dendriticum and B. lobatum.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第5期345-348,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家重大基础研究发展规划"973"项目(G1999011906)~~
关键词
腔阔盘吸虫
双腔科
18S
RRNA
分子分类
Eurytrema coelmaticum
Dicrocoeliidae
18S rRNA
Molecular classification
