摘要
选用水稻品种越光和笹锦为材料,在开花后设置自然温度和高温两种处理,研究了不同温度处理下籽粒蔗糖、果糖和葡萄糖含量的动态变化以及蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性的差异。高温处理下,蔗糖合酶活性值大于转化酶活性值,且与淀粉积累速率呈显著正相关,表明蔗糖合酶在蔗糖的分解和淀粉的合成过程中起着重要作用;高温下两品种籽粒的蔗糖含量明显增加,但分解的葡萄糖和果糖含量并未相应增加,表明高温有利于籽粒中蔗糖的积累,而不利于籽粒中蔗糖的分解。高温下蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性明显下降,表明蔗糖分解速率的下降与蔗糖合酶和转化酶活性下降有关。
The dynamic changes of sucrose, fructose, olar invertase（VCI）, and cell wall bound invertase（CWI） glucose contents and differences in sucrose synthase（SS）, vacuactivities in rice grain after flowering were studied under natural and high temperatures by using two japonica varieties Koshihikari and Sasanishiki as materials. In rice grains, sucrose synthase activity was higher than invertase activity, and positively significantly correlated with starch accumulation rate, indicating that sucrose synthase played an important role in sucrose degradation and starch synthesis. Sucrose content in rice grains increased significantly, whereas fructose and glucose contents did not increase correspondingly under high temperature, suggesting that high temperature treatment enhanced sucrose accumulation, but diminished sucrose degradation in rice grains. Sucrose synthase, vacuolar invertase, and cell wall bound invertase activities in rice grains at high temperature decreased definitely compared with controls, showing that the deceleration of sucrose-degradation rate was related to the decrease of sucrose synthase and invertase activities.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2006年第6期626-630,共5页
Chinese Journal of Rice Science
基金
四川省教育厅重点研究项目(2004A023)
关键词
高温
蔗糖
蔗糖合酶
液泡型转化酶
细胞壁结合型转化酶
水稻
籽粒
high temperature
sucrose
sucrose synthase
vacuolar invertase
cell wall bound invertase
rice
grain
