摘要
【目的】尝试利用转基因方法提高旱稻铁含量。【方法】采用基因枪和农杆菌转化法,将水稻胚乳特异表达的谷蛋白基因启动子GluB-1驱动下的大豆铁结合蛋白基因转入2个优良旱稻品种“旱稻297”和“旱稻277”。利用PCR、Southern杂交分析方法验证转化结果。【结果】转基因植株T1代RT-PCR证明,外源基因在种子中特异表达。T1～T3代的PCR检测证明铁结合蛋白基因已经稳定遗传。多数转基因植株T0～T3代种子中铁含量高于未转化植株,最高达到未转化对照的2倍以上。【结论】利用基因枪和农杆菌转化法可将大豆铁结合蛋白基因导入旱稻基因组中,并使转基因稻米铁含量增高。
[Objective] The objective of this study was to increase the iron content of upland rice using the method of transformation. [Method] We have transferred the soybean ferritin gene into two elite upland rice cultivars （Handao297 and Handao277） using the method of Biolistic and Agrobacterium-mediated transformation. The soybean ferritin gene was expressed under the control of a rice seed storage protein glutelin promoter GluB-1. Transformation results were demonstrated by PCR and Southern blot analysis. [Result] It was confirmed that the soybean ferritin gene was specifically expressed in seeds by RT-PCR analysis and inherited stably by PCR analysis of transgenic T1-T3 generations. Iron content in most of transgenic plant seeds of T0-T3 generations was higher than that of in the control. The maximum iron concentration in the transgenic plants was about two-fold greater than that of in the non-transformant. [ Conclusion] The soybean ferritin gene can be transferred into upland rice using the method of Biolistic and Agrobacterium-mediated transformation, which increased iron concentration in the transgenic plant seeds.
出处
《中国农业科学》
CAS
CSCD
北大核心
2006年第11期2362-2367,共6页
Scientia Agricultura Sinica
基金
国家转基因植物研究与产业化专项资助课题(JY03-B-11)
关键词
早稻
基因枪法
农杆菌法
铁结合蛋白基因
铁含量
Upland rice （Oryza sativa）
Biolistic transformation
Agrobacterium-mediated transformation
Ferritin gene
Ironcontent
