摘要
目的研究山东地区汉族人群HLA-A,-B等位基因的分布特征,探讨中国广大地区甚至世界其它人种利用山东脐血供者进行造血干细胞移植的可能性。方法应用PCR-SSO方法对山东地区5844例无血缘关系的汉族健康新生儿脐带血进行了HLA-A,-B等位基因的分布调查。结果在山东汉族人群HLA-A等位基因中,共检出20种等位基因,其中频率较高的等位基因依次为A02(0.3041),A11(0.1443),A24(0.1434),A30(0.0975)和A33(0.0859);频率较低的等位基因是A34(0.000599),A25(0.000513),A66(0.000513),A74(0.000428)和A36(0.0000856)。在HLA-B等位基因中,共检出46种等位基因,其中频率较高的等位基因依次为B13(0.1348),B51(0.07128),B62(0.0712),B61(0.0676)和B60(0.0642);频率较低的等位基因是B77(0.0000856),B76(0.000171),B47(0.000257),B42(0.000342)和B72(0.000428)。通过山东汉族人群与不同地域的汉族人群的比较显示在不同种族的人群中有其各自独特的人群分布特征,并且在不同地域的同一民族人群中亦有其独特的地理分布特征。结论山东脐血HLA基因具有丰富的多态性,能够代表山东汉族人群HLA的分布特征,进一步反映了北方汉族HLA的分布规律;同时,北方人在山东脐血库中最容易寻找到HLA-A,-B等位基因相合的异基因脐血供者。中国南方人在山东也完全可能寻找到I类等位基因相合的异基因脐血供者,其机率较北方人稍低。
Objective To invesngate the HLA-A,-B allele polymorphism in Shandong Han population and explore the possibility of providing the cord blood donor matched for more patients from larger region of China to perform the stem cell transplantation. Methods 5844 cord blood samples from Shandong Umbilical Cord Bank were investigated by polymerase chain reaction-sequence specific oligonucleotide （PCR-SSO）. Results 20 alleles at HLA-A locus, and 46 alleles at HLA-B locus in Shandong Hans donors were detected. Of 20 HLA-A alleles detected, the most prevalent five alleles were A ＊ 02（0. 3041 ）, A ＊ 11 （0. 1443 ）, A ＊ 24 （0. 1434 ）, A ＊ 30 （0. 0975 ） and A ＊ 33 （ 0. 0859 ） ; A ＊ 34 （0. 000599 ）, A ＊ 25 （0. 000513 ）, A ＊ 66 （0. 000513 ）, A ＊ 74 （0. 000428 ） and A ＊ 36 （ 0. 0000856 ） had lower frequency de- tection. Of 46 HLA-B alleles detected, the most prevalent five alleles were B ＊ 13 （0. 1348）, B ＊ 51 （0. 07128）, B ＊ 62 （ 0. 0712 ）, B ＊ 61 （0. 0676 ） and B ＊ 60 （0. 0642 ） ; HLA-B ＊ 77 （0. 0000856 ）, B ＊ 76 （0. 000171 ）, B ＊ 47 （0. 000257 ）, B ＊ 42 （0. 000342 ） and B ＊ 72 （ 0. 000428 ） showed lower frequency expression. Comparing the HLA-A,-B gene frequency of Shandong Han population with that of other Han Chinese, there were unique distribution of HLA-A, -B alleles among the studied population groups from various regions with the same race origin. The difference from various regions in the same race was less than that among different races. Conclusion There are rich polymorphism of HLA-A,-B in Shandong umbilical cord blood donors, reflecting the distribution characteristics of HLA-A,-B not only in Shandong Han but also in northern Han Chinese. A patient of northern Han Chinese is easier to match a HLA-A, -B cord blood donor in Shandong Umbilical Cord Blood Bank. It is also possible for some patients from southern Chinese to receive a transplant of cord blood stem cell matched with HLA-A, B from Shandong Umbilical Cord Blood Bank.
出处
《中国小儿血液与肿瘤杂志》
CAS
2006年第5期244-247,251,共5页
Journal of China Pediatric Blood and Cancer
关键词
脐血
人类白细胞分化抗原
基因多态性
Umbilical cord blood
Human leukocyte antigen
Genetic polymorphism
