摘要
目的：观察中药溃结饮不同给药途径对大鼠溃疡性生结肠炎结肠黏膜EGF表达水平的影响,揭示口服与灌肠给药结肠黏膜修复的作用特点．获得指导合理选择用药途径,制定综合治疗方案的理论和实验依据．方法：采用TNBS/乙醇法造模,将大鼠随机分为5组,正常组和模型组蒸馏水灌胃,其余分别为奥沙拉秦钠灌胃组,渍结饮灌胃组和灌肠组．采用免疫组化方法及图像分析测定不同给药途径下,结肠黏膜表皮生长因子(EGF)表达的动态变化．结果：药物干预后大鼠溃疡性结肠炎结肠黏膜EGF表达含量增加,溃结饮治疗组别优势明显．急性期溃结饮灌胃后EGF表达量超过溃结饮灌肠组和奥沙拉秦钠组,与灌肠组比较无统计学意义,与奥沙拉秦钠组比较差异有显著性(0．312±0．160 vs 0．217±0．052,P＜0．05)．病程中期药物干预作用不明显．后期中药溃结饮无论在灌胃还是在灌肠方面都显示出其增加黏膜EGF含量的优势,且溃结饮灌肠组优于溃结饮灌胃组,两者比较差异有显著性(0．372±0．132 vs 0．307±0．101．P＜0．05)．结论：渍结饮灌胃起效快,急性期占一定优势；溃结饮灌肠起效较慢,从开始发挥作用直至病程末期都能良好促进黏膜修复．联合应用两种给药方法,急性期和中后期分别以口服和灌肠为主治疗本病,可能会有效缩短黏膜修复时间,加快溃疡愈合．
AIM： To observe the effects of Kui Jieyin given in different ways on the expression of mucosal epidermal growth factor （EGF） in the rectum and colon of rats with ulcerative colitis （UC）, and explore a better method for the treatment of UC.
METHODS： The UC rat model was induced by intermixture of TNBS and ethanol. All the rats were divided into 5 groups. Normal group （n = 15） and model group （n = 15） were given distilled water. The rest rats were intragastrically administered with olsalazine sodium （group A） or Kui Jieyin （group B）, or treated with Kui Jieyin enema （group C）. Then the expression of rectal and colonic mucosal EGF was detected by immunohisthchemical staining.
RESULTS： After drugs interference, the expression of colonic mucosal EGF in UC was increased, especially in group B and C. For the active UC, the expression of EGF was higher in group B than that in group C （no significance） or A （0.312 ±0.160 vs 0.217 ± 0.052, P 〈 0.05）. The drugs had no marked effect on the UC in the middle stage. For the late-stage UC, expression of EGF was significantly increased in group B and C. Furthermore, there was notable difference between group C and B （0.372 ±0.132 vs 0.307 ± 0.101, P 〈 0.05）.
CONCLUSION： Intragastric administration of Kui Jieyin is superior in the acute stage of UC, while Kui Jieyin clyster has constant effect from the beginning to the end of this disease. Combination of these two approaches may accelerate the concrescence of ulcer.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第29期2872-2875,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30572390
国家中医药管理局中医药科学技术研究基金
国中医药科02-03GP03~~
