摘要
目的：探讨脂肪肝大鼠瘦素水平的变化及丹参注射液的影响．方法：♂SD大鼠随机分为5组,其中1组为正常组,服用普通饲料,ip生理盐水；余4组用高脂饲料建立脂肪肝模型后,随机分为模型组、大剂量治疗组、小剂量治疗组、对照组,分别ip生理盐水、大、小剂量丹参注射液、古拉啶溶液．8wk后取血处死大鼠,测定血清瘦素等,观察脂肪肝病理变化．结果：脂肪肝模型大鼠肝指数较正常组升高(3．09％±0．24％vs 2．63％±0．19％,t=4．61,P =0．00),而血清瘦素水平较正常组低下(1．83±0．44μg/L vs 3．04±0．55μg/L,t=5．08,P= 0．00),大剂量丹参注射液提高模型组大鼠血清瘦素水平(2．29±0．46μg/L vs 1．83±0．44μg/ L,t=2．33,P=0．03)．大剂量丹参治疗后脂肪肝大鼠肝组织脂肪沉者面积减少(1．45±0．93 vs 2．40±0．70,t=2．60,P=0．02)．结论：丹参注射液改善脂肪代谢可能通过提高瘦素水平而实现．
AIM： To detect the level of leptin in the nonalcohic fatty liver of rats and the preventive effects of Salvia miltiorrhiza （SM） injection.
METHODS： Male Sprague Dawley rats were selected randomly and given a fat-rich diet for 8 wk. The rats with fatty livers were randomly divided into model group, high-dose SM group, low-dose SM group and reduced glutathione control group. The serum level of leptin was detected by radioimmunoassay （RIA）. The pathological changes in rat livers were observed.
RESULTS： The index of rat nonalcohic fatty liver was significantly higher than that in normal rats （3.09% ± 0.24% vs 2.63% ± 0.19%, t = 4.61, P = 0.00）, while the level of leptin was markedly lower than that in normal rats （1.83 ± 0.44 μg/L vs 3.04 ± 0.55 μg/L, t = 5.08, P = 0.00）. The level of leptin was dramatically elevated while the fat accumulation area in rat livers was decreased after high-dose SM injection as compared with that in model rats （leptin： 2.29 ± 0.46 μg/L vs 1.83 ± 0.44 μg/L, t = 2.33, P = 0.03; area： 1.45 ± 0.93 vs 2.40 ± 0.70, t = 2.60, P = 0.02）. CONCLUSION： SM injection can improve fat metabolism by increasing the level of leptin.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第29期2876-2879,共4页
World Chinese Journal of Digestology
关键词
脂肪肝
瘦素
丹参注射液
大鼠
预防作用
Nonalcoholic fatty liver
Leptin
Salvia miltiorrhiza injection
Rats
