摘要
目的采用基因芯片技术筛选二羟环氧苯并芘(BPDE)转化的人支气管上皮细胞(16HBE)相关基因,探讨该技术在分子毒理学研究中的应用。方法将实验组(BPDE-16HBE)和对照组(16HBE)的mRNA逆转录合成cDNA掺入荧光分子为探针,杂交于H40S基因芯片,分析2组基因的差异表达。结果在4096种人类基因中,BPDE转化的16HBE和正常16HBE间存在差异表达的基因有143条,其中高表达52条,低表达91条。结论基因芯片技术在筛选BPDE转化的16HBE相关基因改变上,具有高通量、高敏感、快速等特点,在毒物的分子致癌机制研究中意义重大。
Objective To screen the differentially expressed genes in human bronchial epithelial call line（16HBE） after treatment with dihydroxyepoxy benzo pyrene（BPDE） by cDNA microarray. Methods 16HBE ceils and 16HBE cells were treated with BPDE only. The total RNAs were extracted from these cells. The eDNA probes were prepared by labeling with Cy3 - dCTP and Cy5 - dCTP respectively through reverse transcription. The mixed probes were then hybridized to the cDNA microarray chips containing 4096 human genes. The chips were scanned by ScanArray 4000 laser scanner. The acquired fluorescent signals were analyzed by GenPix Pro 3.0 software. Bioinformatical analysis of those differentially expressed genes had been performed.Results 143 genes exhibited differential expression between cells treated with BPDE ordy and 16HBE. The expression of 91 genes（63.63 % ） was down - regulated and of 52 genes（36.36 % ） was up - regulated. Conclusion The regulation of genes including stress response genes, immune related genes, DNA synthesis and repair genes MetaboLism genes may be invdved in itransforming activity of BPDE.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第11期1350-1352,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30000138
30200235
30371196
39170651
30271111)
广东省自然科学基金(20011054)
广东省社会发展领域科技计划项目(2004-139-47)
广东省医学科研基金项目(B2002058)
关键词
基因芯片
二羟环氧苯并芘
人支气管上皮细胞
gene ehip
dihydroxyepoxy benzo pyrene（BPDE）
human bronchial epithelial cell（16HBE）
