摘要
目的：建立双氯芬酸胆碱（DAC）高效液相色谱测定方法。方法：采用C18色谱柱，以甲醇-乙腈-磷酸盐（体积比为25：25：50）为流动相，检测波长276nm，进样量20μl。24只家兔，用DAC滴眼液50μl后，取房水用甲醇处理后，取上清液进样测定。结果：本方法线性范围10～500mg／L，r=0．99981，回收率为101．3％，日内和日间RSD均〈5％。家兔用药后，房水中15min既可检测到药物，在给药后30min达到最高浓度。结论：该方法准确灵敏，精密度好，适合于眼房水中DAC的测定。
Aim： To establish an HPLC method for the determination of diclofenac acid-choline （DAC） in aqueous humor of rabbits. Methods： The concentration of DAC in aqueous humor of 24 rabbits treated with DAC eyedrop （50 μl） was determined by HPLC with diamonsil C18 as the chromatographic column, methanol-water-phosphoric acid （25 ： 25 ： 50）as the mobile phase, detection wavelength of 276 nm and injection volume of 20 μl. Results： The linear range was 10-500 mg/L, r =0. 999 81. The mean recovery rate was 101.3%. The intra-day and inter-day RSD were less than 5%. DAC could be determined within 15 min and reached peak concentration at 0.5 h after administration. Conclusion： The method is sensitive and accurate. And it is suitable for DAC ascertainment in aqueous humer.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2006年第6期1085-1086,共2页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目0124180207
