摘要
以大花红景天、云南红景天、长鞭红景天和库页红景天的茎和叶为外植体进行组织培养,结果表明:大花红景天以茎为外植体诱导芽效果最好,其它3种红景天以叶为外植体诱导芽效果最好.云南红景天和长鞭红景天适合的芽诱导激素组合是0.1 mg/L NAA和2.5 mg/L 6-BA的组合,在该激素水平下两种红景天的出芽频率分别达到71%和84%;大花红景天和库叶红景天适合的芽诱导激素组合是0.5 mg/L NAA和2.5 mg/L 6-BA的组合,在该激素水平下两种红景天的出芽频率均达到80%.长鞭红景天和库叶红景天在添加IBA的培养基上容易生根形成完整植株,生根率分别达到87%和73%;经过炼苗后,长鞭红景天再生苗能够成功移栽,成活率达66%.
The stems and leaves of Rhodiola crenulata, Rhodiola yunnanensis, Rhodiola fastigiata and Rhodiola sachalinensis were in vitro cultured as the explants, the stems of Rhodiola crenulata and the leaves of Rhodiola yunnanensis ,Rhodiola fastigiata and Rhodiola sachalinensis were found to be the best parts for shoot induction. The optimal hormone combination for shoot induction of Rhodiola yunnanensis and Rhodiola crenulata was 0.1 mg/L NAA plus 2.5 mg/L 6-BA and the shoot induction percentages of the two species were 71% and 84% with the optimal hormone combination,respectively;The optimal hormone combination for shoot induction of Rhodiola fastigiata and Rhodiola sachalinensis was 0. 5 mg/L NAA plus 2.5 mg/L 6-BA and the shoot induction percentages of the two species reached 80% with the optimal hormone combination;the induced shoots of Rhodiola fastigiata and Rhodiola sachalinensis were easy to root on the medium with IBA added and the rooting percentages were 87% and 73% ,respectively; After having been hardened the regeneration plantlets of Rhodiola fastigiata could be successfully transplanted and survive at the rate of 66 %.
出处
《西北植物学报》
CAS
CSCD
北大核心
2006年第10期2023-2027,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
中国科学院与西藏自治区科技合作项目
关键词
红景天
组织培养
再生植株
Rhodiola
tissue culture
regeneration plantlet
