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小鼠TIM2基因真核表达载体的构建及鉴定 被引量：2

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摘要 目的:构建真核表达载体pIRES2-EGFP-TIM2,并在小鼠肝癌细胞系H22中进行表达。方法:用RT-PCR法扩增得到TIM2基因,构建重组真核表达载体pIRES2-EGFP-TIM2,并进行BamHI及BglⅡ双酶切鉴定和测序。通过脂质体法转染H22细胞,用RT-PCR法检测H22细胞中TIM2mRNA的表达。结果:构建了真核表达载体pIRES2-EGFP-TIM2,用脂质体法转染H22细胞后,用荧光显微镜观察和RT-PCR法检测,可见细胞内有EGFP及TIM2mRNA的表达。结论:成功地构建重组真核表达载体pIRES2-EGFP-TIM2,并在小鼠H22细胞中表达,为进一步研究TIM2在肿瘤生物治疗中的应用奠定了基础。
作者 马玲娣 张彦 文世宏 何於娟 刘小珊 康格非 蒋纪恺
机构地区 南京医科大学附属常州第二人民医院中心实验室 重庆医科大学检验系临床生化教研室 重庆医科大学检验系临床生化教研室 汕头大学医学院分子生物学中心
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2006年第6期713-715,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目(30171150)
关键词 T细胞免疫球蛋白和黏蚩白结构域分子2 增强型绿色荧光蛋白 真核表达载体 小鼠H22肝癌细胞
分类号 R394 [医药卫生—医学遗传学]
  • 相关文献

参考文献8

  • 1Jennifer J,Mclntire,Sarah EU,et al.Identification of Tapr(an airway hyperreactivity regulatory locus)and the linked Tim gene family[J].Nature Immunol,2001,2(12):1109-1117.
  • 2Kumanogoh A,Marukawa S,Suzuki K,et al.Class Ⅳ semaphorin sema4A enhances T-cell activation and interacts with TIM-2[J].Nature,2002,419(6907):629-633.
  • 3Monney L,Sabatos CA,Gaglia JL,et al.Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease[J].Nature,2002,415(6871):536-541.
  • 4朱汉钢,冯作化,耿辉,张桂梅.肿瘤组织中Tim-3表达的特征及其在肿瘤免疫耐受中的作用[J].细胞与分子免疫学杂志,2005,21(4):403-407. 被引量：8
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二级参考文献12

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  • 8Sabatos CA, Chakravarti S, Cha E, et al. Interaction of Tim-3 and Tim-3 ligand regulates T helper type 1 response and induction of peripheral tolerance[ J]. Nat Immunol, 2003, 4:1102 - 1110.
  • 9Sanchez-Fueyo A, Tian J, Picarella D, et al. Tim-3 inhibits T helper type 1-mediated auto-and alloimmune responses and promotes immuno-logical tolerance[ J]. Nat Immunol,2003,4:1093 - 1111.
  • 10Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulate macrophage activation and severity of an auto-immune disease[J]. Nature, 2002, 415:536 - 541.

共引文献7

同被引文献12

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  • 7JENNIFER J, MCLNTIRE, SARAH E UMETSU, et al. Identification of tapr (aft airway hyperreactivity regulatory locus) and the linked tim gene family [J]. Nature Immunology, 2001, 2 (12): 1109-1117.
  • 8ATSUSHI KUMANOGOH, SATOKO MARUKAWA, KAZUHIRO SUAUKI, et al. Class 1V semaphorin sema4A enhances T-cell activation and interacts with TIM-2 [J]. Nature, 2002, 419: 629-633.
  • 9LAURENT MONEY, CATHERINE A SABATOS, JASON L GAGLIA, et al. Th1-speciflc cell surface protein tim-3 regulates macrophage activation and severity of an autoimmune disease [J]. Nature, 2002, 415: 536-542.
  • 10PAUL S, CALMELS B, ACRES RB. Improvement of adoptive cellular immunotherapy of human cancer using ex-vivo gene transfer[J]. Curr Gene Ther, 2002, 2(1): 91-100.

引证文献2

二级引证文献5

细胞与分子免疫学杂志
2006年 第6期

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