摘要
目的探讨外阴鳞状细胞癌(鳞癌)组织中染色体变异与人乳头状瘤病毒(HPV)感染状态的关系。方法应用比较基因组杂交(CGH)技术和PCR技术分别检测21例外阴鳞癌患者癌组织中染色体的变异情况和HPV感染状态,并分析两者间的关系。结果每例患者的外阴鳞癌组织中染色体均有不同程度的变异,包括染色体全部或部分的扩增或丢失,每例患者平均有4·9处(102/21),其中丢失多于扩增,分别为2·6处(54/21)和2·3处(48/21)。21例患者中,根据染色体变异部位的不同,染色体扩增率依次为3q占43%(9/21)、8q占38%(8/21)、12q占33%(7/21)和9p占19%(4/21),染色体丢失率依次为4p占52%(11/21)、3p占43%(9/21)和9p占10%(2/21)。在染色体3q及12q部位,HPV阳性的外阴鳞癌组织染色体扩增率(分别为73%、64%)均明显高于HPV阴性者(分别为10%、0,P<0·01);而在染色体8q部位,HPV阴性的外阴鳞癌组织染色体扩增率(70%)明显高于HPV阳性者(9%,P<0·01)。结论外阴鳞癌组织中存在染色体的扩增或丢失。染色体3q、12q的扩增与外阴鳞癌患者感染HPV有关。
Objective To investigate chromosomal gains and losses in association with human papillomavirus status in the vulvar squamous cell carcinomas (VSCC). Methods We analyzed 21 VSCC with comparative genomic hybridization (CGH) and polymerase chain reaction. Results Each case had different degree of variances, including gains and losses of partial or whole chromosome. On average, each case had 4. 9 ( 102/21 ) abnormal regions; losses were more than gains, being respectively 2. 6 (54/21 ) and 2. 3(48/21 ). Main variations were gains on chromosomes 3q(43% ,9/21 ) ,8q(38% ,8/21 ) , 12q(33% , 7/21 ) and 9p ( 19% ,4/21 ), and losses of 4p ( 52% , 11/21 ) ,3p(43% ,9/21 ) and 9p( 10% ,2/21 ). Gains of 3q(73% ) and 12q(64% ) were significantly more common in human papillomavirus (HPV)-positive VSCC compared to HPV-negative VSCC ( 10% , 0; P 〈 0. 01 ) . Gains on chromosome 8q ( 70% ) were more commonly seen in HPV-negafive compared to HPV-positive VSCC (9%, P 〈 0.01 ). Conclusions Chromosomal gains or losses are detected by CGH in VSCC. The data indicate that gains of 3q and 12q coincide with HPV infection in VSCC.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2006年第10期701-705,共5页
Chinese Journal of Obstetrics and Gynecology
基金
辽宁省自然科学基金资助项目(20042067)
关键词
外阴肿瘤
癌
鳞状细胞
染色体
变异(遗传学)
核酸杂交
乳头状瘤病毒感染
Vulvar neoplasms
Carcinoma, squamous cell
Chromosomes
Variation (genetics)
Nucleic acid hybridization
Papillomavirus infections