摘要
通过观察大鼠脾切除后不同时间及注射大肠杆菌协同作用后血浆TNF水平动态变化过程。结果发现:脾切除后血浆TNF水平升高,其中切脾后5,7夭较明显(P<0.01)。腹腔注射LPS对照组较未注射组血浆TNF水平明显升高。注射LPS组切脾前后血浆TNF水平无明显差异,但均处在较高水平上,提示大肠杆菌内毒素是刺激引起血浆TNF升高的主要原因之一。因此:我们推测脾切除后血浆TNF水平的增高可能系机体免疫力低下及创伤和肠道细菌移位等造成一过性的内毒素血症,以及脾切除后血清内毒素杭体水平降低引起血内毒素水平相对升高引起。这可能是脾切除后凶险性感染(OPSI)发生机制的一个组成部分。
To evaluate the role of tumor necrosis factor in post-splenectomy rats, dy- namic changes in plasma level of tumor necro- sis factor TNFin Wistar rats at different times after splenectomy and insult with E. coli LPS were observed. The TNF level was signif- icantly increased in splenectomized rats than that in control, especially 5 and 7 days after splenectomy P<0.01. This result was simi- lar to the increase of TNF level in groups with E. coli LPS insult. There were no evident dif- ference of TNF level between the LPS insult groups. TNF levels after splenectomy and in- sults showed no significant difference, and they were all at high level. It is postulated that the elevation of plasma TNF might be caused by impairment of immunity of the host and transient endotoxemia resulted from trauma and translocation of intestinal flora, which might be part of pathogenesis of overwhelm- ing post-splenectomy infection.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1996年第6期363-364,共2页
Chinese Journal of Experimental Surgery
