摘要
建立了2，4-二硝基苯肼柱前衍生反相高效液相色谱紫外检测法测定血清中克拉霉素的含量，将在碱性条件下甲基叔丁基醚的血清萃取物，与2，4-二硝基苯肼在55％酸性条件下反应30min，然后用乙腈-0．05mol/LpH7．2磷酸盐缓冲液（48：52）在Alltima C18色谱柱上进行分离，在340nm检测衍生物。方法的线性范围为0．05～3．2mg／L（r=0．9993）；检出限为30μg/L，绝对回收率大于89％，相对标准差小于10％。本方法已用于克拉霉素在健康受试者中的药代动力学研究。
A reversed-phase high performance liquid chromatographic method for the determination of clarithromycin in serum with ultraviolet detection after pre-column derivatization with 2,4-dinitrophenylhydrazine was developed. A ten-butyl methyl ether extract, obtained from serum under alkaline condition, was treated with 2,4-dinitrophenylhydrazine （DNPH） for 30 min at 55° Cunder acidic solution,and chromatographed on an Alltima C18 column using an eluent composed of acetonitrile-0.05 moL/L pH 7.2 phosphate buffer （48 ： 52）. The detection wavelength was set at 340 nm. The linear range for clarithromycin was 0.05 - 3.2 mg/L with correlation coefficient 0.9993. The limit of detection was 30 μg/L, absolute recovery was more than 89%, RSDs were less than 10%. This method has been applied to pharmacokinetic study of clarithromycin in healthy volunteers.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第U09期168-170,共3页
Chinese Journal of Analytical Chemistry
关键词
柱前衍生化
高效液相色谱法
血清
2
4-二硝基苯肼
克拉霉素
Pre-column derivatization, high performance liquid chromatography, serum, 2,4-dinitrophenylhydrazine, clarithromycin
