摘要
目的:探讨外周血细胞CEAmRNA,CK19mRNA水平与胃癌EAP方案介入化疗近期疗效相关意义.方法:选择30例中晚期胃癌患者(Ⅱ期3例,Ⅲ期15例,Ⅳ期12例),于EAP(VP-16+ADM+CBP)介入化疗前2-3d及化疗后2-3wk取外周血,全自动发光免疫法检测血清CEA及TPA,逆转录聚合酶链反应检测CEA及CK19mRNA.用超声内镜(EUS)结合CT测量肿块对化疗的有效率(CR+PR)情况.结果:介入化疗前后外周血CEA及CK19mRNA均有显著差异[CEA:60.0%(18/30)vs33.3%(10/30),χ2=4.29,P<0.05;CK19:73.3%(22/30)vs46.7%(14/30),χ2=4.34,P<0.05].CEA及CK19mRNA联合检测阳性率在介入化疗前后也有显著差异[90.0%(27/30)vs50.0%(15/30),χ2=8.52,P<0.05].影像学诊断显示,外周血CEAmRNA,CK19mRNA联合检测阳性患者治疗前后分别为16例及5例(χ2=8.86,P<0.05).结论:外周血细胞CEAmRNA、CK19mRNA水平在EAP方案介入化疗后阳性率显著下降,CEAmRNA、CK19mRNA联合检测的敏感性高于血清CEA、TPA联合检测,可反应EAP方案介入化疗后肿瘤体积的变化情况.
AIM： To explore the values of carcinoembryonic antigen （CEA） and cytokeratin 19 （CK19） mRNA expression in the evaluation of artery intervention chemotherapy for progressive gastric cancer. METHODS： A total of 30 gastric patients （n = 3, 15, and 12, respectively, for stage II, HI, and IV） were included in this study. Peripheral blood samples were collected 2-3 d before and 2-3 wk after EAP interventional chemotherapy. Auto- mated immunoassay was used to measure the level of serum CEA and tissue polypeptide anti- gen （TPA）, and reverse transcription-polymerase chain reaction was performed to detect the ex- pression of CEA and CK19 mRNA. Endoscopic ultrasound （EUS） combined with computed tomography （CT） was applied to assess the efficacy of chemotherapy.
RESULTS： The expression of CEA and CK19 mRNA in peripheral blood cells were significantly different before and after interventional chemotherapy [CEA： 60.0% （18/30） vs 33.3% （10/30）, χ^2 = 4.29, P 〈 0.05; CK19： 73.3% （22/30） vs 46.7% （14/30）, χ^2 = 4.34, P 〈 0.05]. The positive rate of CEA and CK19 mRNA combined detection was also markedly different before and after chemotherapy [90.0% （27/30） vs 50.0% （15/30）, χ^2 = 8.52, P 〈 0.05]. Imaging diagnosis showed that the positive number of patients received CEA and CK19 mRNA combined detection were 16 and 5 respective, before and after chemotherapy （χ^2= 8.86, P 〈 0.05）.
CONCLUSION： The expression levels of CEA and CK19 mRNA are down-regulated in peripheral blood cells after EAP chemotherapy, and combined detection of them is more sensitive than that of serum CEA and TPA combined detection. Combined detection of CEA and CK19 mRNA can reflect the changes of tumor size after chemotherapy.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第31期3055-3059,共5页
World Chinese Journal of Digestology
关键词
胃肿瘤
逆转录聚合酶链反应
角蛋白19
癌胚抗原
介入化疗
放疗
Gastric carcinoma
Reverse transcription-polymerase chain reaction
Carcinoembryonicantigen
Cytokeratin 19
Znterventional chemotherapy
Radiotherapy
