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蚕豆气孔保卫细胞中的NOS类蛋白定位及其功能分析 被引量：1

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摘要 利用免疫荧光显微镜技术和免疫胶体金标记技术确定蚕豆气孔保卫细胞中存在一氧化氮合酶(NOS)类似蛋白,NOS主要分布在气孔保卫细胞的细胞核、细胞质、叶绿体、线粒体以及细胞壁上.局部灼伤和外源茉莉酸(JA)都能提高蚕豆叶片和表皮NOS活性和一氧化氮(NO)水平,NOS的活性变化与叶片中的NO的变化趋势基本一致;NOS抑制剂L-NAME可抑制JA诱导的NO水平的增加.由此推测,NOS途径是伤胁迫和JA诱导形成NO的主要途径.药理学实验表明适当增加Ca2+浓度能够提高叶片NOS的活性和NO的水平,说明蚕豆叶片NOS活性和NO的分布具有一定的钙依赖性.保卫细胞中NOS及其催化形成的NO可能通过对气孔运动的调节参与植物对逆境的响应.
作者 刘新 王幼群 贾文锁 娄成后 张蜀秋
机构地区 中国农业大学生物学院植物生理学与生物化学国家重点实验室
出处 《科学通报》 EI CAS CSCD 北大核心 2006年第21期2495-2500,共6页 Chinese Science Bulletin
基金 国家自然科学基金(批准号:30370141)资助项目.
关键词 一氧化氮合酶 分布 气孔保卫细胞 钙依赖性 蚕豆
分类号 S643.6 [农业科学—蔬菜学]
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参考文献25

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二级参考文献27

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科学通报
2006年 第21期

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