摘要
在原核表达中影响外源基因表达效率的因素有很多,这其中翻译起始效率起了非常重要的作用。翻译起始效率又主要受SD序列、SD序列与起始密码子之间的间距、DB(DownstreamBox)序列、mRNA翻译起始区(TIR)的二级结构和稀有密码子等因素的影响。主要针对DB序列和5′端稀有密码子的优化设计了软件。通过计算机对序列进行分析比对后,按照匹配碱基数、匹配位置、密码子使用频率平均值的顺序进行排序,给出一些优化序列,并给出了软件算法。
TIR （Translation Initiation Region） efficiency is very important in prokaryotic expression. The TIR＇s efficiency is highly dependent on SD （Shine-Dalgarno） sequence, distance between SD sequence and start codon, DB （Downstream Box） sequence, TIR＇s second structure, codon adaptation and so on. In this paper, we designed and implemented the software to optimize DB sequence and 5＇ rare codons. It generated some optimization sequences by analyzing the target sequence and comparing it with 16S RNA. And the optimization sequences codon adaptation. We drew up the algorithm and the core of is sorting by number of base pairing, location of base pairing and code in this paper.
出处
《生物工程学报》
CAS
CSCD
北大核心
2006年第6期1032-1035,共4页
Chinese Journal of Biotechnology
