期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

利用椭圆小球藻硝酸还原酶缺失突变体为生物反应器表达兔防御素NP-1蛋白 被引量：4

Expression of Rabbit Neutrophile Peptide-1 in Nitrate Reductase-deficient Mutant of Chlorella ellipsoidea
下载PDF
导出
摘要 利用转基因小球藻为生物反应器生产兔防御素NP-1蛋白具有重要的应用价值。本研究利用椭圆小球藻(Chlorellaellipsoidea)硝酸还原酶(nitratereductase)缺失突变体为受体,构建了包含NPTII基因和硝酸还原酶基因两个筛选标记的兔防御素蛋白表达载体,采用电激法将目的基因转入椭圆小球藻硝酸还原酶缺失突变体nrm-4,获得了正确表达防御素蛋白的转基因藻,从而表明通过硝酸还原酶作为筛选标记基因并结合硝酸还原酶缺失突变体可作为较好的小球藻生物反应器生产模式。 Application of transgenic Cholrella as bioreactor to express rabbit neutrophile pepetide-1 （NP-1） shows great practical value. In this paper, an NP-1 expression vector containing two selective marker genes NPTII and nitrate reductase gene was constructed. The NP-1 gene was transformed into the nitrate reductase-deficient mutant nrm-4 of Chlorella ellipsoiclea via electroporation, and the transgenic alga expressed the active NP-1 were obtained.
作者 张小宇 王鹏 赵世民 李霞 沈昕 孙勇如 储成才 王义琴
机构地区 中国科学院遗传与发育生物学研究所 北京林业大学生物科学与技术学院
出处 《遗传》 CAS CSCD 北大核心 2006年第12期1580-1584,共5页 Hereditas（Beijing)
基金 国家十五海洋863青年基金项目(编号:2004AA628010)~~
关键词 椭圆小球藻 硝酸还原酶缺失突变体 兔防御素 Chlorella ellipsoidea nitrate reductase-deficient mutant rabbit neutrophile peptide-1
分类号 Q943.2 [生物学—植物学]
  • 相关文献

参考文献20

  • 1Jarivs E,Brown L M.Transient expression of firefly luciferase in protoplast of the green alga Chlorella ellipsoidea.Curr Genet,1991,19(6):317～321.
  • 2Maruyama M,Horakova I,Honda H.Introduction of foreign DNA into Chlorella saccharophila by electroporation.Biotech Tech,1994,8(1):821～826.
  • 3Dawson H,Burlingame R,Cannons A C.Stable transformation of Chlorella:rescue of nitrate reductase-deficient mutants with the nitrate reductase gene.Curr Microbiol,1997,35(6):356～362.
  • 4Hawkins R I,Nakamura M.Expression of human growth hormone by the eukaryotic alga,Chlorella.Curr Microbiol,1999,38(6):335～341.
  • 5Piers K L,Brown M H,Hancock R E.Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria.Gene,1993,134(1):7～13.
  • 6Lehrer R I,Ganz T,Selsted M E.Defensins:endogenous antibiotic peptides of animal cells.Cell,1991,64(2):229～230.
  • 7Selsted M E,Miller C W,Novotny M J,Morris W L,Koeffler H P.Molecular analysis of myeloperoxidase deficiency shows heterogeneous patterns of the complete deficiency state manifested at the genomic,mRNA,and protein levels.Blood,1993,82(4):1317～1322.
  • 8Murphy C J,Foster B A,Mannis M J,Selsted M E,Reid T W.Defensins are mitogenic for epithelial cells and fibroblasts.J Cell Physiol,1993,155(2):408～413.
  • 9Lichtenstein A,Ganz T,Selsted M E,Lehrer R I.In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes.Blood,1986,68(6):1407～1410.
  • 10Ganz T,Selsted M E,Szklarek D,Harwig S S,Daher K,Bainton D F,Lehrer R I.Defensins.Natural peptide antibiotics of human neutrophils.J Clin Invest,1985,76(4):1427～1435.

二级参考文献55

  • 1Hancock R E W,Diamond G.The role of cationic antimicrobial petitides in innate host defensin[J].Microbiology,2000,8(9):402-410.
  • 2Yang D A,Biragyn L,Kwak W,et al.Mammalian defensins in immunity:more than just microbicidal[J].Trends Immuno,2002,23(16):291-296.
  • 3Haynes R J,Tighe P J,Dua H S.Expression of human beta-defensin-1 promote differentiation of keratinocytes[J].Br J Ophthalmol,1999,183(6):737-741.
  • 4Frye M ,Bargon J, Gropp R.Antimicrobial petitides of the human oculat surface.[J].J Mol Med,2001,79(5-6):275-282.
  • 5Jia H P, Schutte B C,Schudy A,et al .Discovery of new human β-defensins using a genomics based approach[J].Gene,2001,263(1-2):211-218.
  • 6Garcia J R ,Krause A ,Schulz S ,et al .Human L-defensin-4:a novel inducible petitide with a special salt-sensitive spectrum ofantimicrobial activity[J].FASEB J,2001,15(10):1819-1821.
  • 7Diamond G,Kaiser V,Rhodes J,et al.Transcriptional regulation of β-defensin gene expression is trchel epithelial cells[J].Infect Immun,2000,68: 113-122.
  • 8Bals R,Goldman M J,Wilson J.Mouse β-defensins-1 is a salt-sensitive antimicrobial petitide present in epithelia of the lung and urogenital tract[J].Infect Immun,1998,63(3):1225-1232.
  • 9Mizukawa N,Sugiyama K,Yamachika E,et al.Presence of defensin in epithelial langerhans cell adjacent to oral carcinomas and precancerous lesions[J].Anticancer Res,1999,19(4B):2969-2971.
  • 10Mizukawa N,SugiyamaK,Ueno T,et al.Levels of human defensin-1,an antimicrobial petitide,in saliva of patientswith oral inflamnation[J].Oral Surg Oral Med Oral Patbol Oral Rbdiol Endod,1999,87(5) 539-543.

共引文献19

同被引文献185

引证文献4

二级引证文献33

遗传
2006年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部