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跨膜型血管内皮生长因子受体1在子痫前期患者胎盘组织中的表达及其意义 被引量：1

Expression of fms-like tyrosine kinase receptor 1 in placenta of pre-eclampsia
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摘要 目的研究跨膜型血管内皮生长因子受体1(Flt-1)在子痫前期患者胎盘组织中的表达变化及其意义。方法采用RT-PCR方法检测20例子痫前期患者(观察组)胎盘组织中Flt-1mRNA的相对表达量[以Flt-1与β肌动蛋白(β-actin)电泳条带吸光度(A)值的比值表示],并以20例正常孕妇(对照组)为对照;采用蛋白印迹(westernblot)法检测和比较两组各18例胎盘组织中Flt-1的蛋白相对表达量[其余4例标本因蛋白提取不理想而废弃,以Flt-1与磷酸甘油醛脱氢酶(GAPDH)电泳条带A值的比值表示]。结果两组孕妇胎盘组织中均有Flt-1的表达,观察组孕妇胎盘组织Flt-1mRNA相对表达量为2·25±0·19,对照组为1·23±0·29,两组比较,差异有统计学意义(P<0·05);观察组孕妇胎盘组织Flt-1蛋白相对表达量为2·67±1·19,对照组为0·94±0·51,两组比较,差异也有统计学意义(P<0·05)。结论Flt-1可能参与了子痫前期的病理生理过程。 Objective To investigate the expression of fins-like tyrosine kinase receptor 1 （ Flt-1 ） in placentas of pre-eclampsia. Methods The expression of Flt-1 mRNA in the placentas from 20 preeclampsia patients and 20 pregnant women with normal blood pressure was detected by semi-quantitative reverse transcription-polymerase chain reaction. The protein expression of Flt-1 was analyzed using western blot in 18 pre-eclampsia patients and 18 normotensive pregnant women. Results Placental Flt-1 mRNA level in pre-eclampsia was 2. 25 ± 0. 19 （ intensity ratios of Fh-1 mRNA to β-actin mRNA） , significantly higher than in normotensive pregnant women 1.23± 0. 29 （ P 〈 0. 05 ）. Western blot showed that Flt-1 protein level in pre-eclamptic placenta was 2. 67 ± 1. 19 [ western blot signal intensity ratios of Flt-1 to glyceraldehyde-3-phosphate dehydrogenase （GAPDH） ] , significantly higher than in pregnant women with normal blood pressure 0. 94 ± 0. 51 （ P 〈 0. 05 ）. Conclusion Increased Flt-1 expression in pre-eclamptic placenta may be involved in pathogenesis of pre-eclampsia.
作者 侯秀红 李东红 李怡 赵宏喜 张春利 黄亮 姚元庆
机构地区 第四军医大学唐都医院妇产科
出处 《中华妇产科杂志》 CAS CSCD 北大核心 2006年第11期736-739,共4页 Chinese Journal of Obstetrics and Gynecology
基金 国家自然科学基金资助项目(30070787)
关键词 先兆子痫 胎盘 血管内皮生长因子受体1 Pre-eclampsia Placenta Vascular endothelial growth factor receptor-1
分类号 R714.2 [医药卫生—妇产科学]
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参考文献17

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二级参考文献31

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共引文献17

同被引文献11

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中华妇产科杂志
2006年 第11期

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