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RNA干扰survivin表达对喉癌细胞系Hep-2对紫杉醇敏感性的影响 被引量：1

Effects of laryngeal cancer cell line Hep-2 to paclitaxel sensitivity induced by RNAi survivin gene
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摘要 目的探讨运用RNA干扰技术阻断喉癌细胞系Hep-2中survivin基因的表达,并研究survivin基因沉默后Hep-2对紫杉醇敏感性的影响。方法化学合成21bp目的基因siRNA片段,并将不同浓度的siRNA用脂质体法转染喉癌细胞系Hep-2。用空脂质体和GFP-siRNA作平行对照。通过蛋白印迹法检测survivin表达量的变化,MTT法检测转染后Hep-2细胞对紫杉醇敏感性的变化。结果25nM和50nM的siRNA可以有效地阻断了Hep-2细胞survivin蛋白的表达;转染后Hep-2细胞在体外对紫杉醇的敏感性增加。结论初步阐明survivin在喉癌细胞对紫杉醇敏感性中的作用,为进一步探讨survivin基因与喉癌的关系以及联合治疗研究奠定了基础。 Objective To study the effects of survivin silencing by RNAi in laryngeal cancer cell line Hep2 on taxol chemosensitivity. Methods Specific RNAi fragment directed to surviving was chemically generated. RNAi fragments of various concentrations were transfected into Hep2 cells, Survivin expression in transfected cells were analyzed by Western blot to verify the silencing efficiency, Taxol chemosensitivity was detected by MTT assay. Results Survivin expression level was effectively suppressed by RNAi in a dose-dependent manner. The minimal effective dose was 25 nM. Hep2 cells of silenced survivin were more sensitive to taxol treatment. Conclusion This study showed that survivin played an important role in taxol sensitivity. Suppressing survivin offers a novel means to enhance chemosensitivity in laryngeal carcinoma.
作者 关中 薛红漫 冯公侃 邹华
机构地区 中山大学第二附属医院耳鼻喉科
出处 《国际医药卫生导报》 2006年第21期4-5,共2页 International Medicine and Health Guidance News
基金 广东省自然科学基金(31696) 广东省卫生厅(B2003036)
关键词 RNA干扰 SURVIVIN 喉癌 RNA interference survivin laryngeal cencer cells paclitaxel
分类号 R739.65 [医药卫生—肿瘤]
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参考文献7

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二级参考文献11

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同被引文献11

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国际医药卫生导报
2006年 第21期

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