摘要
目的观察栀子提取物ZG对单纯疱疹病毒1型感染后宿主细胞膜流动性的影响,以探讨其抗病毒作用机理。方法采用NBD-C6-HPC特异性荧光探针标记Hep-2细胞膜脂质,以肝素钠为阳性对照,借助激光共聚焦扫描技术检测栀子提取物ZG对病毒感染后宿主细胞膜流动性的影响。结果正常细胞膜具有良好的流动性,漂白后荧光恢复率为73．89％；病毒感染后Hep-2细胞膜流动性显著降低,荧光恢复率为18．54％；栀子提取物ZG作用后细胞膜流动性明显恢复,荧光恢复率为61．21％；肝素钠作用后细胞膜流动性明显恢复,荧光恢复率为56．62％。结论通过改善细胞膜的流动性,从而维持细胞膜的正常功能可能是栀子提取物ZG抗病毒感染的机理之一。
Objective To analyze the effect of Gardenia extract ZG on the membrane fluidity of Hep-2 cells infected by herpes simplex virus type 1 and explore the mechanism of its antiviral activity. Methods With heparin sodium as control, the membrane phosphohpid of Hep-2 labelled with NBD-C6-HPC was examined under confocal laser scanning microscope（CLSM） to explore the change of membrane fluidity taking place after HSV-1 infection. Results The membrane fluidity of normal Hep-2 cells was good, with a recovery rate of 73.89% after photobleaching. It decreased significantly after HSV-1 infection, with a recovery rate of 18.54%. When the cells were treated with Gardenia extract ZG or heparin sodium after HSV-1 infection, the membrane fluidity showed a significant recovery, with recovery rates of 61.21% and 56.62%, respectively. Conclusion Cardenia extract ZG can improve membrane fluidity and protect the cell membrane, which may be one of the mechanisms explaining its anti-HSV-1 activity.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第11期1005-1008,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金面上项目(30371754)
