摘要
目的:观察胰岛素样生长因子-1(IGF-1)基因转染骨髓基质细胞(MSCs)与鸵鸟钙磷陶瓷骨(OTBC)相复合后,MSCs在OTBC上的生长状态。方法:培养大鼠MSCs,以脂质体介导IGF-1基因转染MSCs后,G418筛选体外扩增后,通过免疫组化鉴定获得稳定表达细胞克隆,传代与OTBC复合,不同时间点用扫描电镜观察。结果:免疫组化证实IGF-1基因转染MSCs高表达IGF-1,复合OTBC后生长良好,24h细胞已贴附、伸展,随着时间的增加,细胞生长与增殖明显。结论:IGF-1基因转染MSCs在OTBC上生长良好,IGF-1基因转染MSCs体外三维立体培养模型有望应用于牙周组织工程。
Objective：To evaluate the biocompatibility of insulin-like growth factor 1 （IGF-1） gene transfected bone marrow stem cells （MSCs） with ostrich true bone ceramic （OTBC）. Methods：Rat MSCs were transfected with IGF-1 gene, and positive clones were selected by （3418. The expression of IGF-1 protein in the MSCs was detected by immunocytochemical technique. The IGF-1 transfected MSCs were cultured with OTBC and the morphology of the cells was observed by scanning electronic microscope（SEM） at different time point. Results：Immunohistochemical staining suggested that the IGF-1 protein was expressed in the IGF-1 transfected MSCs. The cells adhered to OTBC and stretched well after 24 h of culture. The IGF-1 transfected MSCs proliferated on the surface of OTBC with culture time. Conclusion：The OTBC has a good biocompatibility with IGF-1 transfected MSCs.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第6期838-840,共3页
Journal of Practical Stomatology
关键词
骨髓基质细胞
胰岛素样生长因子-1
鸵鸟钙磷陶瓷骨
扫描电镜
Bone marrow stem cells
Insulin-like growth factor l
Ostrich true bone ceramics
Scanning electronic microscope
