摘要
目的:观察topiramate对宫内急性脑缺血损伤的Wistar大鼠神经细胞凋亡及脑组织含水量的影响。方法:夹闭足月妊娠大鼠子宫血管,制成急性脑缺血损伤的新生鼠模型(n=315),随机分为topiramate 1次和5次给药组及缺血组(n=105),另取105只正常Wistar大鼠作为正常对照组。治疗组给予topiramate,观察脑缺血后再灌注3 h、6 h、24 h、3 d、7 d、14 d、21 d、28 d海马TUNEL法标记的凋亡神经细胞的变化及生后7 d内脑组织含水量的变化。结果:topiramate 1次组脑组织含水量12和72 h显著低于缺血对照组,topiramate5次组48和72 h显著低于缺血对照组及topiramate 1次组(P<0.05);topiramate 1次组24 h、3 d、7 d、21 d凋亡神经细胞数明显低于缺血对照组,topiramate 5次组3和7 d凋亡神经细胞数明显低于缺血对照组和topiramate1次组(P<0.05)。结论:topiramate可以明显减少宫内急性脑缺血后新生大鼠神经细胞的凋亡数目,并可以缩短凋亡持续时间;延缓脑水肿的发生,减轻脑水肿的程度,并缩短其持续时间,多次给药组的作用明显高于1次给药组。
Objective To investigate the effects of topiramate on neurons apoptosis and the hydrous capacity in brain tissue in rats with perinatal acute ischemic brain damage. Methods The perinatal acute ischemic brain damage model was established by ligation of both uterine arteries of full-term pregnant Wistar rats （n = 315）. They were divided randomly into topiramate one and five times groups and hypoxic-ischemia （HI） group （n= 105）, and normal collate group consisted of 105 normal Wistar rats . The effects of topiramate were observed by the changes of hippocampal apoptosis cells labeled in situ end TUNEL methods at different time spots （postnatal 3 h, 6 h, 24 h, 3 d, 7 d, 14 d, 21 d, 28 d） and the hydrous capacity in brain tissue within 7 d after birth. Results The hydrous capacity in brain tissue in administering drug one time group was remarkably less than that in HI group at 12 and 72 h after birth, and it in administering drug five time group was remarkably less than those in HI group and administering drug one time group at 48 and 72 h after birth （P〈0.05）. The number of apoptosis neurons in administering drug one time group was remarkably less than those in HI group 24 h, 3 d, 7 d, 21 d after birth, it in administering drug five time group was remarkably less than those in HI group and administering drug one time group 3 and 7 d after birth （P〈0.05）. Conclusion Topiramate can remarkably lessen the degree of neurons apoptosis and shorten the duration of apoptosis; delay the occurrence of cerebral edema after HI, lessen the degree of cerebral edema, and shorten the duration of cerebral edema. The protective nerve function of topiramate in the many times administering drug group is obviously higher than that in the one time administering drug group.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第6期1041-1044,F0003,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省杰出青年基金资助课题(20050113)
吉林大学青年基金资助课题(2005年)
教育部高等学校博士学科点专项基金资助课题(20040183066)
