摘要
目的：进行黄连Coptis chinensis种质资源的遗传多样性研究。方法：对32份黄连种质进行ISSR分析。利用TREECONW软件分析遗传相似系数，UPGMA方法聚类，构建亲缘关系系统图。结果：16条引物共得到106条扩增条带，其中有51条呈现多态性，占48．1％。遗传相似系数变化范围0．8258～0．9351。聚类结果显示黄连种质亲缘关系与地理分布无明显相关性，但可以看出来源于同一地区的部分黄连种质聚在一起，呈现出一定的地域性分布规律。结论：栽培黄连的遗传多样性水平较低，种质问的亲缘关系较近。
Objective： To detemine the genetic diversity of Coptis chinensis. Method： 32 gennplasmie resources of C. chinensis were analyzed by ISSR molecular markers. To make up the systematic diagram of genetic relationship by TREECONW software and clustered by UPGMA method. Result： A total of 106 ISSR bands were scored, among which 51 were polymorphic bands. The average percentage of polymorphic bands was 48. 1%. Genetic similarity coefficient were changed from 0. 825 8 to 0. 935 1. By cluster analysis, the geographical distribution is not very obvious, but it was also showed some of the C. chinensis from the same region were in the same group which presented the law of geographical distribution in the tested materials. Conclusion： Different gerrnplasms diversity of C. chinensis is low and the relationship of C. chinensis is close.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第23期1937-1940,共4页
China Journal of Chinese Materia Medica
基金
国家科技攻关计划(2004BA721A32)
重庆市科技计划项目(8847)
关键词
黄连
ISSR种质资源
遗传多样性
Coptis chinends
ISSR
germplasmie resources
genetic diversi
