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茄子反转录转座子基因片段的克隆及序列分析

Cloning and Sequencing of a Retrotransposon Gene Fragment from Eggplant
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摘要 根据己报道的反转录转座子的序列设计合成一对特异引物,以西安绿茄基因组DNA为模板,采用PCR扩增的方法扩出一条DNA特异片段并克隆到pMD18-T载体中.用PCR法和酶切分析法对克隆片段进行鉴定并进一步进行核苷酸序列分析.序列测定该片段长为518 bp.用GenBank中的BLAST程序分析表明,该片段与马铃薯(Solanum demissum)反转录转座子的gag-pol聚合蛋白90-243aa区域氨基酸同源性为43%. PCR primers were designed depend on the reported sequences of plant retrotransposon. Xi＇an green eggplant was used as experimental material. A DNA fragment was amplified by polymerase chain reaction and cloned into the vector pMD18 - T, which was confirmed by PCR and enzyme digesting. Sequence measurement showed that the cloned fragment was 518bp in length. The results of searching the GenBank by blast methods showed that this sequence and Solanum demissum putative gag-pol polyprotein were 43 % homologous in region from 90aa to 243aa.
作者 赵海艳 孙清鹏 秦勇 赵福宽
机构地区 北京农学院生物技术系 新疆农业大学园艺学院
出处 《中央民族大学学报（自然科学版）》 2006年第4期299-303,共5页 Journal of Minzu University of China(Natural Sciences Edition)
基金 北京市自然科学基金资助项目(项目编号:5982006)
关键词 茄子 反转录转座子 克隆 eggplant retrotransposon cloning
分类号 S641.1 [农业科学—蔬菜学]
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参考文献14

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中央民族大学学报（自然科学版）
2006年 第4期

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