摘要
目的评价转铁蛋白-多聚乙烯亚胺(Tf-PEI)靶向性转染小鼠骨肉瘤细胞的可行性。以Tf-PEI为载体,将小鼠白细胞介素(IL)-12基因导入小鼠骨肉瘤细胞。方法以Tf-PEI为载体,将绿色荧光蛋白基因和小鼠IL-12基因分别导入小鼠骨肉瘤细胞,并检测其转染效率。以游离转铁蛋白竞争性拮抗Tf-PEI,并观察它对转染效率的影响。结果Tf-PEI可以高效的、靶向性的转染小鼠骨肉瘤细胞。当N／P比值为5时,Tf-PEI的转染效率最高。游离转铁蛋白能够显著的拮抗Tf-PEI的转染。Tf-PEI还可以成功的将小鼠IL-12基因导入小鼠骨肉瘤细胞。结论Tf-PEI是一种高效率、低毒性、肿瘤靶向性的基因转染载体,可以广泛应用于恶性肿瘤靶向性基因治疗的试验。
Objective To investigate the feasibility and efficiency of transferrin-polyethylenimine （Tf-PEI） for tumor-targeted gene transfer to murine osteosarcoma. Using Tf-PEI as the vector, to transfer murine interleukin-t2 （mIL-t2） gene into mouse osteosarcoma cells. Methods Using Tf-PEI as the vector, murine osteosarcoma cells were transfected with plasmid DNA coding for murine IL-12 and green fluorescent protein （GFP）,and the expression of the transduced gene was detected. By using free transferrin to inhibit Tf-PEI, the influence on the transfection of Tf-PEI was observed. Results Tf-PEI could effectively and specifically deliver murine IL-12 gene and luciferase gene into murine osteosarcoma cells. When N/P ratio was 5 ,the transfection efficiency of Tf-PEI was highest. The transfection can be specifi- cally inhibited by free transferrin. Conclusion Tf-PEI is a high efficiency and low cytotoxicity tumortargeted vector. It can be widely used in the area of tumor-targeted gene therapy.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第12期1519-1521,共3页
Chinese Journal of Experimental Surgery
