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胆固醇酯酶高产菌株的筛选及动力学性质研究 被引量:6

Screening of cholesterol esterase-producing strain and study on enzyme kinetics
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摘要 通过平板初筛和摇瓶复筛,得到一株胆固醇酯酶的高产菌株,研究了pH值、温度、底物浓度、一些有机和无机试剂对胆固醇酯酶酶促反应速度的影响。研究发现,胆固醇酯酶有较好的酸碱稳定性,pH值为5.0 ̄8.0;最适pH值和最适温度分别为pH7.0和40℃,该酶的动力学研究表明,对胆固醇亚油酸酯和胆固醇油酸酯都表现为典型的米氏规律,Km值分别为2.2×10-5mol/L3.1×10-5mol/L,同时研究了一些金属离子和有机化合物对酶活性的影响,发现Mg2+、EDTA对胆固醇酯酶有激活作用。 A high-yield cholesterol esterase-producing strain of Rhodococcus sp. was obtained with plate method and shake flask fermentation. The effects of pH, temperature, substrate concentration and organic/inorganic reagents on the velocity of cholesterol esterase (COE) were inve-stigated. The enzyme was stable over a broad range of pH 5.0-8.0. The optimum pH and temperature were 7.0 and 40℃, respectively. The behavior of cholesterol esterase was in accord with Michaelis-Menten kinetics when cholesterol linoleate or cholesterol oleate was used as substrate, with a Km of 2.2 × 10^-5mol/L or 3.1 × 10^-5mol/L, respectively. The effects of metal ions and organic compounds on enzyme activity were also studied. It was found that Mg^2+and EDTA could activate the cholesterol esterase.
出处 《中国酿造》 CAS 北大核心 2006年第12期36-39,共4页 China Brewing
关键词 胆固醇酯酶 RHODOCOCCUS sp. 动力学特性 cholesterol esterase Rhodococcus sp. kinetic property
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参考文献5

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同被引文献65

  • 1杨光礼,陈壁佩,张一闵,秦克亮,熊振平.假单胞菌胆固醇酯酶的生产Ⅰ.菌种筛选和发酵条件的研究[J].医药工业,1987(3):112-115. 被引量:2
  • 2黄武华,方定一,崔玉兰,方一澄.假单胞菌1299产胆固醇酯酶的研究 Ⅰ.适宜的产酶条件[J].微生物学通报,1989,16(5):274-277. 被引量:1
  • 3陈文祥,董军,唐蔚青,李培瑛,李健斋.高效液相色谱评价血清胆固醇试剂中酶的性能[J].中华医学检验杂志,1995,18(4):208-210. 被引量:4
  • 4张德超 王素英 王翠英.转化胆固醇的微生物在食品中的应用研究.中国食品学报,1999,(11):85-126.
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