摘要
以8个中国地方绵羊品种和1个外来品种的20个个体为研究对象,通过对Cytb基因的全序列测定,结果表明:绵羊的单倍型多样性为97.1%。所有序列的平均碱基组成为27.1%T,28.5%C,31.4%A及13.0%G,G+C含量为41.5%,核苷酸多样性为0.602%。在所有序列中共检测到43个变异位点,其中包括40处转换和3处颠换。Fu’s中性检验表明差异不显著(0.10>P>0.05),说明绵羊群体未发生群体扩张事件。NJ、ME及UPGMA聚类结果均表明,我国绵羊可分为3个单倍型组,这提示我国绵羊有3个母系起源。
The complete sequences of Cyt b gene from 20 individuals belonging to eight Chinese indigenous sheep breeds and one foreign breed were studied. The results showed that the hapolotype diversity of Chinese sheep breeds was 97.1%. The mean nucleotide composition of all the sequences was 27.1% T, 28.5% C, 31.4% A, and 13.0% G. The nucleotide diversity was 0.602%. A total of 43 mutation sites were detected, including 40 transitions and 3 transversions. Fu＇s test of selective neutrality showed that the sheep populations had no population demographic expansion （0.10 〉 P 〉 0.05）. The different clustering methods, namely neighbor-joining, minimum evolution, and unweighted pair group method with arithmetic means, all showed a similar result, which indicated that Chinese local sheep had three maternal resources.
基金
This work was supported by the National Natural Science Foundation of China (No. 30371026), Doctor Startup Fund (No. 01140503) and Topnotch Person with Personal Ability Support Project of Northwest A&F University (No. 01140101).
关键词
绵羊
CYT
6
系统进化
sheep
Cyt b
phylogenetic evolution
