摘要
目的：建立血浆、羊水、母乳及胎盘等多种生物医学样品中铜、铁、锌等微量元素的水平ICP—OES分析法。方法：优化了仪器操作参数，包括射频功率、提升量、雾化器压力等，样品经HNO3-HClO4湿式消解后进行测定。结果：测定了200多份新生早产儿和足月儿脐血与母血血浆及胎盘、羊水等样品中的铜、铁、锌等微量元素的含量，方法准确度以加标回收率表示，为95．0％～102．8％，精密度RSD（n=5）为0．2％～2．7％，方法检出限符合要求。结论：本法快速简便、灵敏、准确，结果令人满意。
Objective： A procedure for the determination of K, Na, Ca, Cu, Fe, Zn, Mg, Mn, Cr and Se in biomedical samples by axial view inductively coupled plasma optical emission spectrometry （ICP- OES） has been described. Methods： Instrumental operating parameters have been optimized. The samples were wet digested by HNO3 - HClO4 before determination. Results ：The contents of micro- elements in premature birth newborn baby and mature fetus of umbilical blood, mother＇s blood, placenta, mother＇s latex and ammiotic fluid were determined. The accury expressed as recovery rate was 95.0% - 102. 8% and the precision RSD（n = 5） 0. 2% - 2. 7%. The limit of detection accorded with the demand. Conclusion： The method is simple, rapid, accurate and sensitive.
出处
《中国卫生检验杂志》
CAS
2006年第12期1415-1417,1436,共4页
Chinese Journal of Health Laboratory Technology
