摘要
目的了解下呼吸道感染肺炎克雷伯菌超广谱β-内酰胺酶(ESBLs)和质粒AmpC酶的产生情况及耐药性,研究AmpC酶的基因型别。方法用纸片扩散确证法检测ESBLs;用酶提取三维试验检测AmpC酶,聚合酶链反应(PCR)扩增AmpC酶的基因,DNA序列测定检测AmpC酶的基因型;K-B法检测细菌耐药性。结果58株下呼吸道感染肺炎克雷伯菌中ESBLs阳性21株,AmpC酶阳性5株,其中3株ESBLs和AmpC酶均阳性,5株AmpC酶阳性菌中,4株扩增出DHA基因,经测序均为DHA-1,1株扩增出MIR基因。产酶菌株的耐药性明显高于非产酶株。结论肺炎克雷伯菌中ESBLs和AmpC酶均有较高的检出率,AmpC酶以DHA基因型为主。产ESBLs和AmpC酶是肺炎克雷伯菌耐药的主要原因。
Objective To investigate the resistance AmpC β -lactamase in K. pneumoniae in lower piratory tract and prevalence of extended-spectrum β-lactamase and infection, and to study the genotyping of AmpC β-lactamasc, Methods Disc diffusion confirmatory test was used to detect ESBLs, three dimensional test was used to detect AmpC ,PCR was used to determine the genotype of AmpC, DNA sequencing were performed to determine the sub-types of AmpC β-lactamase,antimicrobial susceptibility was tested by Kirby-bauer method. Results Among the 58 K. pneumoniae strains in lower respiratory tract infection, there were ESBLs in 21 strains, AmpC in 5 strains, ESBLs and AmpC simultaneously occurred in 3 strains. Among the 5 AmpC positive stains, there were DHA-1 in 4 strains, MIR in 1 strains. β-lactamases positive strains were more resistant to antibiotics than β-laetamases negative strains. Conclusions ESBLs and AmpC in K. pneumoniae have a high detectable rate,and DHA-1 was the main genotyping of AmpC. Producing ESBLs and AmpC enzyme was a prominent cause of resistance in K. pneumoniae.
出处
《中国微生态学杂志》
CAS
CSCD
2006年第6期474-476,共3页
Chinese Journal of Microecology
