摘要
在国际β-葡聚糖酶法测定β-葡聚糖含量的基础上,用纤维素酶代替昆布多糖水解酶水解β-葡聚糖,用β-葡聚糖酶代替β-葡萄糖苷酶,用苯酚-硫酸法代替葡萄糖试剂盒法测定β-葡聚糖酶解后得到葡萄糖含量,设计了适合我国应用的β-葡聚糖酶法测定方法。此法测定过程为：1．5mL浓度为60μg/mL的标准β-葡聚糖和一定浓度的样品,用浓度为50U/mL纤维素酶0．5mL酶解60min；然后用蒸馏水稀释至30mL,从中吸取0．5mL到另一试管,再加入浓度为2U/mLβ-葡聚糖酶1mL,作用15min后,用苯酚-硫酸法测定标准β-葡聚糖和样品酶解液中葡萄糖含量,计算出样品中β-葡聚糖的含量。
The determination method applicable to our country was established on bassis of enzymes by substitution of Lichenase with cellulose enzyme, β-glucosidase with glucanase and glucose assay kit with phenol-sulphuric acid method. The procedure of the new enzymatic method was： 1.5mL β-glucan （60μg/mL） and sample were hydrolyzed separately with 0.5mL cellulose enzyme（50U/mL） for 60min, diluted to 30ml with distilled water, 0.1mL β-glucanase （2U/mL） was added into 0.5mL hydrolysate,15min later, glucose content of hydrolysates from standard β- glucan and sample was determined by phenol- sulphuric acid method, content of β- glucan in sample was calculated out.
出处
《食品工业科技》
CAS
CSCD
北大核心
2006年第11期188-191,共4页
Science and Technology of Food Industry
基金
天津商学院引进人才科研启动费基金资助(050102Q)
