摘要
目的:用贵州小香猪建立动脉粥样硬化动物模型,观察小型猪动脉粥样硬化发生发展与血浆C-反应蛋白水平的关系。方法:实验于2003-02/2004-03在南华大学心血管病研究所实验室进行。选择10只贵州小香猪单纯随机分为两组(n=5):①高脂高胆固醇组:喂饲高脂高胆固醇饲料2个月后麻醉状态下切开右侧颈总动脉放置6F动脉球囊导管,压力600～800kPa×180s,重复2次,然后在200～400kPa压力下从远端向近端拖拉球囊导管,并重复3次,术后饲以高脂高胆固醇饲料。②正常对照组:普通饲料喂养2个月后麻醉状态下分离右侧颈总动脉,但不进行血管内膜损伤。实验进行12个月,每2个月检测1次血浆总胆固醇、三酰甘油和高密度脂蛋白胆固醇水平和血浆C-反应蛋白水平,第12个月末处死动物取主动脉和冠状动脉,观察动脉粥样硬化斑块和脂质条纹发生情况。结果:10只小香猪全部进入结果分析。①高脂高胆固醇组小香猪血浆总胆固醇、高密度脂蛋白胆固醇水平从2个月末显著高于正常对照组(P<0.05),而血浆三酰甘油水平从4个月末开始升高,到6个月末才明显增加(P<0.05)。②高脂高胆固醇组小香猪血浆C-反应蛋白水平4个月末时即高于正常对照组(P<0.05),到6个月末明显高于正常对照组(P<0.05)。③在高脂高胆固醇组动物的主动脉、颈总动脉、髂动脉和冠状动脉可见动脉粥样硬化斑块和脂质条纹,该病变区占整个主动脉面积的(90.8±8.3)%;正常对照组未见斑块和脂质条纹。结论:采用血管内膜损伤法加高脂高胆固醇饲料喂养小型猪可建立动脉粥样硬化动物模型。动脉粥样硬化小型猪血浆C-反应蛋白水平升高,C-反应蛋白和动脉粥样化形成相关。
AIM： To establish animal models of atherosclerosis in minipigs and investigate the change of plasma C-reactive protein level in atherosclerotic minipigs. METHODS： The experiment was performed in the Laboratory of Cardiovascular Disease of Nanhua University from Feburary 2003 to March 2004. Ten minipigs were selected and randomly divided into 2 groups with 5 minipigs in each group：①High fat and high cholesterol （HFHC） group： rats were fed with HFHC diet and cut open the left common carotid artery （CCA） under anesthetized after 2 months to place a 6F artery Foley＇s tube at the pressure of 600-800 kPa×180 s for twice. The artery Foley＇s tube was pulled from the distal end to the proximate at the pressure of 200-400 kPa for three times, and pigs were fed with HFHC diet after operation.② Normal control group： pigs were fed with normal food for 2 months and then isolated of the right CCA under anesthetization without endangium injury. The experiment was continued for 12 months, and the levels of plasma total cholesterol （TC）, high-density lipoprotein cholesterol （HDL-C） and triglycerides （TG） were detected, and the plasma CRP concentration was measured once every other month. Animals were executed at the end of the 12^th month to obtain the arteriae aorta and coronary artery. The atherosclerotic plaque and lipid stripe were studied. RESULTS： A total of 10 pigs were involved in the analysis of results.① The levels of plasma TC and HDL-C in the HFHC group were obviously higher than the normal control group from the end of the 2^rd month （P 〈 0.05）, while the level of TG began to ascend at the end of the 4^th week, which significantly increased from the end of the 6^th month （P 〈 0.05）.② The level of plasma CRP in pigs of HFHC group was higher than that in the normal control group since the end of the 4^th month （P〈0.05）, which was obviously higher than the normal control group at the end of the 6^th month （P 〈 0.05）. ③Atherosclerotic plaques and lipid stripes were detected in the aorta, arteria iliaca, arteria carotis communis and coronary artery of animals in the HFHC group, and the diseased region accounted for （90.8±8.3）% of the total aera of aorta. However, no atherosclerotic plaques or lipid stripe was found in the normal control group. CONCLUSION： The animal models of atherosclerosis can be established by integrated method of endangium injury and HFHC diet. The level of serum CRP in atherosclerotic minipigs is increased and there are correlations between CRP and the progression of atherosclerosis.
出处
《中国临床康复》
CSCD
北大核心
2006年第48期77-79,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(30470720)
中国博士后科学基金资助项目(2005037157)~~
