期刊文献+

Targeting Gene-Virotherapy of Cancer and its prosperity 被引量:32

Targeting Gene-Virotherapy of Cancer and its prosperity
下载PDF
导出
摘要 Gene and viral therapies for cancer have shown some therapeutic effects, but there has been a lack of real breakthrough. To achieve the goal of complete elimination of tumor xenograft in animal models, we have developed a new strategy called Targeting Gene-Virotherapy of Cancer, which aims to combine the advantages of both gene therapy and virotherapy. This new strategy has produced stronger anti-tumor effects than either gene or viral therapy alone. A tumorspecific replicative adenovirus vector, designated as ZD55, was constructed by deletion of the 55kDa E1B region of adenovirus. The resulting viral construct not only retains a similar function to ONYX-015 by specifically targeting p53 negative tumors, but also allows for the insertion of various therapeutic genes to form appropriate ZD55 derivatives due to the newly introduced cloning site, a task not feasible with the original ONYX-015 virus. We showed that the anti-tumor effect of one such derivative, ZD55-IL-24, is at least 100 times more potent than that of either ZD55 virotherapy or Ad-IL-24 gene therapy. Nevertheless, complete elimination of tumor mass by the use of ZD55-1L-24 was only observed in some but not all mice, indicating that one therapeutic gene was not sufficient to "cure" these mice. When genes with complementary or synergetic effects were separately cloned into the ZD55 vector and used in combination (designated as the Dual Gene Therapy strategy), much better results were obtained; and it was possible to achieve complete elimination of all the xenograft tumor masses in all mice if two suitable genes were chosen. More comprehensive studies based on this new strategy will likely lead to a protocol for clinical trial. Finally, the concept of Double Controlled Targeting Virus-Dual Gene Therapy for cancer treatment, and the implication of the recent progress in cancer stem cells are also discussed.
出处 《Cell Research》 SCIE CAS CSCD 2006年第11期879-886,共8页 细胞研究(英文版)
关键词 targeting therapy cancer therapy gene-virotherapy 基因治疗 肿瘤 病理 研究
  • 相关文献

参考文献6

二级参考文献48

  • 1郭明高,姜明红,杨琴,李月敏,崔贞福,李林芳,吴孟超,钱其军.腺病毒介导的全抗体基因治疗卵巢癌的实验研究[J].中华医学杂志,2004,84(14):1147-1151. 被引量:16
  • 2唐云霞,陈瑜,顾锦法,齐荣,邹卫国,蒋琳兰,刘新垣.Ad.TERT-TRAIL对肿瘤细胞的杀伤作用及其机理[J].癌症,2005,24(5):536-542. 被引量:5
  • 3Li G Sham J Yang J Su C Xue H Chua D Sun L Zhang Q Cui Z Wu M Qian Q.Potent antitumor efficacy of an E1B 55kDa-deficient adenovirus carrying murine endostatin in hepatocellular carcinoma[J].第二军医大学学报,2005,26(11):1330-1330. 被引量:9
  • 4Kirn D,Martuza R L,Zwiebel J.Replication-selective virotherapy for cancer:Biological principles,risk management and future directions[J].Nat Med,2001,7(7):781-787.
  • 5Zhao L,Gu J,Dong A,et al.Potent antitum or activity of oncolytic adenovirus expressing mda-7/IL-24 for colorectal cancer[J].Hum Gene Ther,2005,16(7):845-858.
  • 6Liu X Y,Qiu S B,Zou W G,et al.Effective gene-virotherapy for complete eradication of tumor mediated by the combination of hTRAIL (TNFSF10) and plasminogen k5[J].Mol Ther,2005,11(4):531-541.
  • 7Pei Z,Chu L,Zou W,et al.An oncolytic adenovrial vector of smac increases antitumor activity of TRAIL against HCC in human cells and in mice[J].Hepatology,2004,39(5):1371-1381.
  • 8Zhang Z,Zou W,Wang J,et al.Suppression of tumor growth by oncolytic adenovirus-mediated delivery of an antiangiogenic gene,soluble Flt-1[J].Mol Ther,2005,11(4):553-562.
  • 9Bischoff J R,Kirn D H,Williams A,et al.An adenovirus mutant that replicates selectively in p53-deficient human tumor cells[J].Science,1996,274(5286):373-376.
  • 10Mineta T,Rabkin S D,Yazaki T,et al.Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant gliomas[J].Nat Med,1995,1(9):938-943.

共引文献77

同被引文献76

引证文献32

二级引证文献69

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部