摘要
目的研究N-硝基-L-精氨酸甲酯(L-NAME)对大鼠实验性隐睾生精细胞凋亡及凋亡相关基因Bax表达的影响。方法手术建立大鼠单侧隐睾模型,实验分为隐睾组、隐睾+L-NAME组[术后腹腔注射溶于生理盐水的L-NAME 50 mg/(kg.d)]、隐睾+生理盐水组、假手术组,7 d后采用化学比色法检测睾丸组织NOS活性,流式细胞术(FCM)和原位缺口末端标记法(TUNEL)检测生精细胞凋亡,RT-PCR和免疫组织化学法检测Bax基因表达变化。结果隐睾组和隐睾+生理盐水组之间检测指标无明显差异;隐睾+L-NAME组隐睾凋亡细胞百分比和生精细胞凋亡指数均低于隐睾组及隐睾+生理盐水组,高于假手术组(P<0.05);Bax mRNA和蛋白表达水平隐睾组及隐睾+生理盐水组最高,假手术组次之,隐睾+L-NAME组最低。结论隐睾生精细胞Bax表达增加,L-NAME可通过下调Bax的表达抑制隐睾生精细胞凋亡。
Objective To investigate the effect of N^w-nitro-L-arginine-methybester（L-NAME） on spermatogenic cell apoptosis and the expression of Bax in rats experimental cryptorehidism. Methods Unilateral cryptorehidism was surgically induced in the rats under pentobarbital anaesthesia. The rats of cryptorchid ＋ normal saline group and cryptorchid ＋ L-NAME group were injected intraperitoneally normal saline and L-NAME [ 50mg/（kg·d）] accordingly. The activity of NOS in the left testis was measured by colorimetric method on the 7th day after operation. The effect of L-NAME on apoptosis of spermatogenic cell in the unilateral cryptorehidism was evaluated by TUNEL and FCM . The mRNA and protein expression level of Bax in testes was detected by RT-PCR and immunohistochemieal assay accordingly. Results Administration of L-NAME could attenuate the activity of NOS and inhibit spermatogenic cells apoptosis in rats experimental cryptorchidism, and it could significantly inhibit weight loss of the cryptorchid. The mRNA and protein expression level of Bax in the cryptorchid testes treated with L-NAME was the lowest among the four groups, and in the testes of the cryptorchid group and the cryptorchid ＋ normal saline group the mRNA and protein expression level of Bax was higher than that of sham operation group. Conclusion The mRNA and protein expression level of Bax in rats experimental cryptorchidism increases significantly. Administration of L-NAME can inhibit spermatogenic cell apoptosis by decreasing Bax expression.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第6期710-714,共5页
Acta Anatomica Sinica
基金
湖南省科委资助项目(03SSY3106)
