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禽呼肠病毒P10、P17非结构蛋白基因的克隆及序列分析 被引量:3

Cloning and Sequnce Analysis of the P10 and P17 Nonstructural Protein Gene of Avian reovirus
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摘要 根据GenBank上的禽呼肠病毒(ARV)S1基因序列,设计并合成了一对跨越P10和P17非结构蛋白基因的特异性引物,对13个ARV毒株进行RT—PCR扩增、克隆及序列测定。结果显示,13个ARV毒株的P10蛋白基因ORF全长均为297bp,编码98个氨基酸;P17蛋白基因ORF全长为441bp,编码146个氨基酸。这13个ARV毒株P10、P17蛋白基因核苷酸同源性分别在96.6%~100%和95.2%~99.3%之间,推导的氨基酸同源性分别在98.2%~100%和91.9%~99.0%之间。将这13个ARV毒株与Gen Bank上其他正呼肠病毒毒株,包括番鸭株(DRV)和飞狐上分离到的内尔森海湾病毒(Nelson Bay Vir US,NBV)及两个澳洲分离株(ARM—1和SOM-4)进行同源性比较和遗传进化树分析,结果表明,呼肠病毒有地域和种类的差别。 A pair of specific primers were designed and synthesized according to S1 gene sequences of Avian reovirus from GenBank, which included the whole open reading frame (ORF) of P10 and P17 nonstructral protein genes. Both P10 and P17 protein genes of 13 ARV strains were amplified,cloned and sequenced. The whole ORF of P10 and P17 protein genes of those 13 ARV strains were 297bp and 441bp in length, respectively. And they were predicted to encode P10 and P17 nonstructral proteins of 98 and 146 amino acids, respectively. The percentages of nucleotide and amino acid sequence identities were determined. The nucleotide and amino acid sequence identities of P10 protein gene among those 13 ARV strains exhibited 96.6%-100% and 91.9%-99.0%, respectively. The identities of P17 protein gene showed 98.2%-100% and 95.2%-99.3% , respectively. Comparison of the nucleotide and predicted amino acid sequences and phylogenetic analysis between ARV and other orthoreoviruses(DRV, NBV, RAM-1,SOM-4) showed that Reo virus had the divergence among regions and species.
出处 《动物医学进展》 CSCD 2006年第12期70-74,共5页 Progress In Veterinary Medicine
基金 广西留学回国人员基金项目(桂科回0144014和0342006) 广西科技攻关项目(023500124)
关键词 禽呼肠病毒 P10、P17非结构蛋白基 克隆 序列分析 Avian reovirus P 10, P 17 nonstructural protein genes cloning sequence analysis
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参考文献9

  • 1谢芝勋,廖敏,庞耀珊,谢志勤,邓显文,刘加波,李康然.禽呼肠孤病毒S1基因的扩增克隆与鉴定[J].中国兽医科技,2000,30(8):3-5. 被引量:17
  • 2Grande A,Costas C,Benavente J.Subunit composition and conformational stability of the oligomeric form of the avian reovirus cell-attachment protein δC[J].Journal of General Virology,2002,83:131-139.
  • 3Wickramasinghe R,Meanger J,Eniguez C E.Avian reovirus proteins associated with neutralization of virus infectivity[J].Virology,1993,194(2):688-696.
  • 4谢芝勋,邓显文,刘加波,庞耀珊,唐小飞,廖敏.禽呼肠孤病毒广西分离株σ_3基因的克隆和表达[J].中国预防兽医学报,2005,27(3):167-170. 被引量:18
  • 5Bodelon G,Labrada J,Martinez C,et al.Modification of late membrane permeability in avian reovirus infected cells[J].The Journal of Biological Chemistry,2002,277:17789-17796.
  • 6Ellis M N,Eidson C S,Brown J,et al.Studies on interferon induction and interferon sensitivity of avian reovirus[J].Avian Dis,1983,27:927-936.
  • 7Costas J M,Lopez G V,Nakharia N,et al.Possible movement of the double-stranded RNA-binding core protein A in the resistance of avian reovirus to interferon[J].J Virol,2000,74:1124-1131.
  • 8Xie Z,Fadl A A,Girshick T,et al.Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction[J].Avian Diseases,1997,41:654-660.
  • 9Hsu H W,SU H Y,Huang P H,et al.Sequence and phylogenetic analysis of P10-and P17-encoding genes of avian reovirus.Avian Dis,2005,49(1):36-42.

二级参考文献9

  • 1Xie Zhizun,Avian Dis,1997年,41卷,654页
  • 2BW.卡尔尼克.高福 苏敬良 译.禽病学[M],第10版[M].北京:中国农业大学出版社,1999.902-908.
  • 3Dale R, pedro K H. Kleven S. Characteristics and pathogenicity of two avian reoviruses isolated from chickens with leg problems[J].Avian Diseases 1983: 27(1) : 255-660.
  • 4NI Y, kenp M C. A comparative study of avian reovirus pathogenicity: virus Spread and replication and induction or lesion [J]. Avian Disease, 1995, 39: 554-566.
  • 5NI Y, Ranting R F, Kemp M C. identification of Proteins encode by avian reoviruses and evidence for posl-trouslational modification[J]. Virology, 1993,193: 466-469.
  • 6Meanger J, Wickramasinghe R: Association between the protein of avian reovirus and titus induced fusion of cell [J]. Archives of virology, 1999, 144: 193-197.
  • 7Shapouri M R, Kane M, Letarte M, et al. Cloning, Sequencing and expression of the S1 gene of avian reovints[J]. Gen Virol, 1995,76,1515-1520.
  • 8谢芝勋,庞耀珊,刘加波,谢志勤,邓显文.应用反转录聚合酶链反应检测鸽Ⅰ型副粘病毒的研究[J].中国兽医科技,2000,30(1):7-9. 被引量:9
  • 9廖敏,谢芝勋,刘加波,庞耀珊,邓显文,谢志勤,唐小飞.鸡呼肠孤病毒分离与鉴定[J].中国家禽,2002,24(1):12-14. 被引量:22

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