摘要
用单一碳源培养结合ERIC-PCR的方法,比较研究了不同程度富营养水中鸭跖草根系细菌数量和组成的变化。结果显示,在8种单一碳源培养基平板上,不同程度富营养水中鸭跖草根系的细菌数量均明显高于对照,其中重度富营养水和轻度富营养水鸭跖草根系细菌的优势菌群分别为利用淀粉碳源的细菌类群和利用山梨醇碳源的细菌类群。ERIC-PCR指纹图谱分析进一步显示,不同程度富营养水中鸭跖草根系样品在相同单一碳源培养基上回收菌群的ERIC-PCR图谱明显不同,表现为条带的数目、位置和亮度的变化,表明其同一生理类群但菌群的组成却各不相同。综合以上结果,可以初步判断不同程度富营养水中鸭跖草根系的细菌生理类群无论在数量或是结构组成上均明显不同。
Bacterial quantity and component changes around rhizosphere of common dayflower （ Cornrnelina cornrnuhis） were comparatively studied which were cultured under eutrophic water of different degree using mono - carbon source media by means of ERIC-PCR methods. The results showed that cultured on the plate of eight kinds of monocarbon source media growing the rhizospheric bacterial population of common dayflower with eutrophic water of two different degrees were obviously larger than the control ones. Among them the bacterial populations of superiority around rhizosphere of common dayflower cultured under eutrophic water of heavy degree and fight degree were utilized starch as carbon source and sorbitol as carbon source respectively. Analysis of ERIC-PCR fingerprint atlas indicated further that the ERIC-PCR atlas of recovered bacterial population of rhizospheric samples from common dayflower in eutrophic water of different degrees which were cultured under the same mono-carbon source was obviously different, performed as the changes on strip number, position and brightness, suggested that the same physiological populations yet their bacterial population components were all different. Summing up the results mentioned above, it could be judged initially that bacterial physiological population of rhizosphere of common dayflower in eutrophic water of different degrees were all obviously different whatever in quantity or in structural components.
出处
《微生物学杂志》
CAS
CSCD
2006年第6期36-39,共4页
Journal of Microbiology
基金
杭州师范学院重点科研项目(2002XNZ02)
关键词
富营养化水
植物根系
细菌生理类群
eutrophic water
plant rhizosphere
bacterial physiological population
