摘要
目的探讨沙尘暴细颗粒物(PM2.5)对大鼠肺细胞的遗传损伤作用。方法采用气管直接注入染毒法,分别给大鼠灌注0,1.5,7.5,37.5 mg/kg剂量的颗粒物悬浮液。灌注24 h后处死大鼠,采用单细胞凝胶电泳技术(SCGE)检测肺细胞DNA的损伤。结果沙尘暴和正常天气PM2.5均可引起大鼠肺细胞DNA损伤,且随剂量增加而损伤增大。正常天气PM2.5比沙尘暴PM2.5对肺细胞DNA的损伤更大,但差异无统计学意义。结论沙尘暴和正常天气PM2.5均可引起大鼠肺细胞DNA的损伤,由于沙尘暴PM2.5浓度远高于正常天气PM2.5,导致沙尘暴PM2.5对DNA的毒性远大于正常天气PM2.5。
Objective To investigate damaging effects of dust storm fine particle （PM2.5） on DNA in lung cells of rats. Methods Wistar rats were randomly divided into toxicosis groups and control group by intratracheal instillation. After instilled 24 hours, DNA damage of lung cells of rats was detected with single cell gel electrophoresis technique. Results Both dust storm PM2.5 and normal weather PM2.5 could lead to DNA damage in a dose - dependent manner. Though the effects induced by normal weather PM2.5 heavier than dust storm PM2.5, there was no significant difference between them. Conclu-sion Both dust storm PM2.5 and normal weather PM2.5 could lead to DNA damage in lung cells of rats. However, the dust storm PM2.5 whose airborne concentrations were much higher than that of normal weather PM2.5, so the dust storm PM2.5 should be more harmful.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第12期1458-1459,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(3023031020477023)
山西省自然科学基金(20031092)
关键词
沙尘暴PM2.5
肺
单细胞凝胶电泳技术
dust storm PM2.5
lung
the single cell gel electrophoresis technique
