摘要
目的:研究丙烯酰胺慢性染毒后睾丸生殖细胞DNA损伤与p53蛋白表达的关系,为丙烯酰胺生殖毒性及其修复机制研究提供基础数据。方法:昆明种小鼠经丙烯酰胺(20、40、60 mg/kg体重)慢性染毒8周后,单细胞凝胶电泳研究睾丸生殖细胞的DNA损伤;免疫组化采用SABC法。结果:丙烯酰胺染毒8周后的各组受损细胞增加,形似彗星;40、60 mg/kg组的细胞拖尾率及尾长显著高于阴性对照(P<0.05);60 mg/kg组的细胞中呈现较多棕黄色颗粒,p53表达在20、40、60 mg/kg各组的阳性率分别为18.1%、21.2%和29.8%,与阴性对照比较60 mg/kg组阳性率有显著差异(P<0.05)。结论:丙烯酰胺有明确的DNA损伤作用,p53蛋白参与了损伤细胞的修复或凋亡途径。
Objective： To investigate the relationship between DNA damage and p53 expression after acrylamide exposure of mice. Methods： Single cell gel electrophoresis was used to determine the DNA damage. P53 expression was probed with immunohlstochemical assay of SABC. Results： Comet tails were observed in the positive controls and all the acrylamide exposure groups. The comet cell rate and tail length in 40 mg/kg and 60 mg/kg b. w. groups were significantly higher than that of negative group （P〈 0.05）. p53 expression was significantly higher in 60 mg/kg b. w. groups than that of negative control （P〈0.05）. Conclusion： It suggested that acrylamide took a definite DNA damage to testes cells from mice. P53 played a role in the apoptosis of testis cells after acrylamide exposure.
出处
《现代预防医学》
CAS
北大核心
2006年第12期2272-2273,共2页
Modern Preventive Medicine
