摘要
自某农药厂污水处理系统的活性污泥中分离纯化一株高效降解3-氯代苯甲酸的菌株S—7,形态学、生理生化特性及16S rRNA基因序列的结果表明S—7为红平红球菌(Rhodococcus erythropolis).研究了在不同温度条件下,R.erythropolisS—7对3-氯代苯甲酸的降解能力.实验表明该菌在20℃和30℃时,于60 h内将底物全部分解.并且分析了该菌在30℃时的反应动力学.在10℃的低温条件下,能以3-氯代苯甲酸为唯一碳源和能源生长,并在5 d内完全降解底物.该菌的耐冷性能对于低温地区生物修复有重要意义,在低温地域有广泛的应用前景.研究了在以葡萄糖为生长基质的条件下,菌株S—7对2-氯代苯甲酸和4-氯代苯甲酸的降解能力.S—7可共代谢2-氯代苯甲酸,但是不能共代谢降解4-氯代苯甲酸.质粒提取实验表明,降解3-氯代苯甲酸的基因位于染色体上.
A strain S-7 which can degrade 3-chlorobenzoate was isolated from activated sludge of a pesticide wastewater treatment plant. Based on its phenotype, chemotaxonomie properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The degradation ability at different temperatures was investigated, The strain could totally degrade 3-ehlorobenzoate within 60 h at 20 ℃ and 30 ℃. The biodegradation kinetics at 30 ℃was investigated. Moreover, the strain could grow at 10 ℃ , using 3-ehlorobenzoate as sole carbon and energy sources, and completely degrade it within 5 d. The psychrotolerant ability was significant for bioremediation in low temperature regions, When glucose was added as grow substrate, the strain S-7 can cometabolicly degrade 2-chlorobenzoate, but cannot biodegrade 4-chlorobenzoate. Plasmid purification showed that the degradation gene lied in the chromosome .
出处
《天津大学学报》
EI
CAS
CSCD
北大核心
2006年第12期1428-1433,共6页
Journal of Tianjin University(Science and Technology)
关键词
生物降解
氯代苯甲酸
低温微生物
共代谢
biodegradation
chlorobenzoate
psychrotolerant bacteria
cometabolism
