摘要
目的探讨种植窗期子宫内膜雌、孕激素受体(ER、PR)及血管内皮生长因子(VEGF)的表达与体外受精-胚胎移植(IVF-ET)结局的关系,选择能预测IVF-ET结局的良好而可靠的指标。方法2004-05-2005-01对郑州大学第二附属医院生殖中心40例拟行IVF-ET妇女,在IVF-ET前1周期采用免疫组化SP法和组织学积分H-score方法对ER、PR和VEGF在种植窗期子宫内膜中的表达进行定位和半定量分析。按IVF-ET后是否妊娠将40例不孕患者分为两组:妊娠组和未妊娠组。结果未妊娠组腔上皮、腺体PR表达较妊娠组强,差异有统计学意义(P<0·05),间质PR表达较妊娠组弱,差异有统计学意义(P<0·05);妊娠组和未妊娠组子宫内膜ER表达差异无统计学意义(P>0·05);VEGF在子宫内膜中的表达妊娠组较未妊娠组高,差异有统计学意义(P<0·05)。结论种植窗期子宫内膜腔上皮、腺体PR下调失败,间质PR表达减弱及子宫内膜VEGF的表达减弱可导致IVF-ET妊娠失败。种植窗期子宫内膜PR和VEGF的表达可作为预测妊娠是否成功的指标。
Objective To study the relationship of endometrial estrogen receptor （ ER）, progesterone receptor （PR） and vascular endothelial growth factor （VEGF） in the window of implantation and pregnancy result of in vitro fertilization and embryo transfer and to select the indexes which can be used to predict IVF-ET result. Methods The endometrium tissue samples were obtained from 40 patients undergoing IVF-ET in the window of implantation. The endometrial ER, PR and VEGF were detected by immunohistochemistry using monoclonal antibody and semi-quantified by using the H- score. Forty patients were divided into two groups： the pregnancy group （16 patients） and non-pregnancy group （24 patients）. Results The endometrial luminal epithelial and glandular epithelial PR score in non-pregnancy group was significantly higher than that in the pregnancy group （ P 〈 0. 05 ）. The endometrial stromal PR score in non-pregnancy group was significantly lower than that in the pregnancy group （ P 〈 0. 05 ）. The endometrial ER score was not significantly dif- ferent in the two groups. The endometrial immunostaining VEGF score was higher in pregnancy group than that in nonpregnancy group （ P 〈 0.05）. Conclusion The failure of down-regulation of the endometrial luminal epithelial, glandular epithelial PR, the decreased stromal PR and the low expressions of endometrial VEGF in the window of implantation can result in IVF-ET failure. The expressions of endometrial PR and VEGF in the window of implantation can be used to predict IVF-ET result.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2006年第12期918-920,共3页
Chinese Journal of Practical Gynecology and Obstetrics
关键词
妊娠
雌
孕激素受体
血管内皮生长因子
子宫内膜
Pregnancy
Estrogen receptor
Progesterone receptor
Vascular endothelial growth factor
Endometrium
