摘要
目的:建立大鼠脑组织蛋白质双向电泳技术。方法:利用不同体积的裂解液提取大鼠脑组织中的蛋白质,并通过不同的蛋白质上样量(1mg,2mg,3mg),进行双向电泳,考马斯亮蓝染色,图谱分析。结果:在等电点3～10,分子量6.5～200ku范围内分离得到蛋白质斑点为,1mg蛋白质上样量为546个蛋白质斑点,2m g为780个,3m g为805个斑点。2mg蛋白质上样量的双向电泳图谱更清晰,分离更好。结论:成功建立了大鼠脑组织蛋白质的双向电泳技术。
Objective： To establish a two-dimensional electrophoresis technology on brain tissue protein of rat.Methods：Lysis buffer of different volume was taken to extract brain tissue proteins of rat, and different protein quantities （1mg,2mg,3mg） were taken to establish a two-dimensional electrophoresis. Coomassie brilliant blue was applied to stain protein,and patterns were analyzed. Results：With a molecular mass between 6.5-200ku and isoelectric points （pI） from 3-10 ,lmg proteins obtained 546 protein spots,2mg 780,and 3mg 805. Pattern of 2mg protein was the best.Concision： A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully.
出处
《重庆医科大学学报》
CAS
CSCD
2006年第6期794-797,共4页
Journal of Chongqing Medical University
基金
重庆市重大科技专项资助课题[渝科发计字(2004)27号]
关键词
蛋白质组学
双向电泳
脑组织
裂解液
Proteomics
Two-dimensional electrophoresis
Brain tissue
Lysis buffer
