摘要
目的光化学法制备一系列不同粒径的聚乙烯亚胺纳米凝胶(polyethylenimine,PEI),并研究其在小鼠体内对组织器官的氧化损伤情况及其影响因素。方法PEI单体在紫外光照射下引发聚合,生成粒径分布较窄且粒径可控的高分子纳米凝胶,并表征。腹腔给予不同剂量的PEI,化学比色法测定小鼠肝脏、肾脏和肺脏组织内GSH和MDA含量及SOD活力。结果光相干光谱仪检测,PEI纳米凝胶粒径为38nm^200nm;扫描电子显微镜和原子力显微镜下多为球形。大剂量时肝脏、肾脏和肺脏组织的GSH和MDA含量及SOD活力均与对照组有明显差异(P<0.05),且MDA随PEI的粒径增大而增高,GSH、SOD含量则有相反的趋势;小剂量时各组织器官的GSH和MDA含量及SOD活力与对照组无差别(P>0.05)。结论大剂量时PEI对小鼠组织器官可造成氧化损伤,PEI的粒径增大其损伤也增加;小剂量分次给予时不明显。MDA的测定和GSH、SOD的测定相互配合,可反映PEI对机体组织器官产生的氧化损伤。
Objective To synthesize polyethylenimine（PEI） nanogels and observe the oxidizing damage and other effects caused by polyethylenimine（PEI） in mice organ. Methods We synthesized polyethylenimine （PEI） nanogels in the range of 80 - 200nm with narrow size distribution by photo-chemistry at room temperature in aqueous solution. The size of nanogels, size distributions and zeta potentials were determined by photo correlation spectroscopy （ PCS）. Spherical morphology of the nanogels was characterized by scanning electron microscopy （SEM） and confirmed by atomic force microscopy （ AFM）. Mice were injected through abdominal cavity with a series of PEI nano-particles which possesed different diameters using high and low dosages. Glutathione（GSH） Maleic Dialdehyde（MDA） and Superoxidedismutase（SOD） in hepar, kidney and lung of these mice were detected through chemo-chromatometry. Results Compared with the control groups, high dosage groups（ 100mg/kg body weight,0.2ml, once/day） displayed more MDA and little GSH SOD（ P 〈 0.05）, while low dosage groups（Smg/kg body weight, 0.2ml, once/day, continuous three days） displayed no different （ P 〉 0.05 ）. Moreover, MDA had an uptrend when PEI diameters increased, GSH and SOD were reverse. Conclusion PEI could cause oxidizing injure in mice organ when using high dosage but no damage were produced when using low dosage and for several times. MDA associated with GSH and SOD was efficient to demonstrate the oxidizing damage caused by PEI.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2006年第6期733-738,共6页
Chinese Journal of Biomedical Engineering
基金
江苏省教育厅自然科学基金资助课题(03KJD320230)。
