摘要
目的探讨外周血T淋巴细胞免疫功能与医院感染发生之间的关系。方法采用流式细胞术对外周血T淋巴细胞进行亚群分析,采用微生物培养鉴定法对医院感染进行监测,回顾性研究了医院感染患者入院初期外周血T淋巴细胞亚群及T4/T8淋巴细胞比值的变化。结果医院感染组病例外周血成熟T淋巴细胞、T4淋巴细胞、T8淋巴细胞的百分率及T4/T8淋巴细胞比值依次为(45.99±12.99)%(、18.80±5.54)%、(19.98±10.62)%和(1.31±1.14)%,其成熟T淋巴细胞、T4淋巴细胞、T8淋巴细胞的百分含量明显低于健康对照组(P<0.01),T4/T8淋巴细胞比值无明显变化(P>0.05)。进一步分组统计显示,包括T4/T8正常亚组、T4/T8倒置亚组和T4/T8升高亚组的各医院感染组,均存在不同程度的T淋巴细胞分布异常和T4/T8比值异常。结论入院初期患者外周血T淋巴细胞免疫功能低下是发生医院感染的重要原因,该结论可为医院感染的预防提供新的思路。
Objective To assess the relationship between nosoeomial infection and immunological function of T lymphoeytes in peripheral blood. Methods Lymphocyte immunologic grouping was detected by flow cytometery. The occurrence of nosocomial infection was examined by a series of microbiological culture and identification methods. The changes of T lymphoeytes subgroup and the ratio of T4 and T8 was investigated by retrospective study. Results The percentages of mature T lymphocyte, T4 lymphocyte and T8 lymphocyle and the ratio of T4and T8 in NI group were （45.99±12.99） %,（18.80±5.54） %,（19.98±10.62） % and （1. 31 ± 1.14）% respectively. The percentages of mature T lymphocyte, T4 lymphocyte and T8 lymphocyte in NI Group were lower clearly than that in health control group＇（P〈0.01）. But the ratio of T4 and T8 had no significant changes. Divided NI group into subgroup, there were abnormalities in amount of mature T lymphocyte and its subgroup distribution as well as the ratio of T4 and T8 including T4/T8 normality subgroup, inversion subgroup and increase subgroup. Conclusion To early admission patients, immunologic functions loss of T lymphocyte in peripheral blood is the important reason resulting in the occurrence of NI. This conclusion maybe offers a new ideal to control NI.
出处
《重庆医学》
CAS
CSCD
2006年第23期2124-2125,共2页
Chongqing medicine
关键词
医院感染
T淋巴细胞
免疫分型
免疫功能
nosocomial infections
T lymphoeytes
immunity grouping
immune function
