摘要
目的:探讨db/db自发性糖尿病小鼠颌下腺神经生长因子、C-Fos蛋白表达与其形态学改变的关系。方法:取3、4、6、8、10月龄db/db(单基因遗传自然发病)型糖尿病小鼠颌下腺(实验组)及相应月龄的db/+m正常小鼠颌下腺(对照组)。采用SP免疫组化染色,观察颌下腺NGF、c-fos阳性表达的变化。结果:随着糖尿病发展,颌下腺实质组织萎缩,细胞缩小,形态不规则,排列不整齐,间质纤维增生,各月龄糖尿病小鼠颌下腺C-fos阳性细胞明显低于相应对照组(P<0.01),NGF阳性细胞明显低于相应对照组(P<0.01),且逐渐减少,呈下降趋势。且逐渐减少,呈下降趋势。结论:颌下腺颗粒曲管细胞合成和分泌NGF功能降低,与糖尿病性神经病变的发生与发展密切相关。db/db糖尿病状态下颌下腺细胞表达c-fos蛋白明显降低,c-fos低表达可能与颌下腺实质的萎缩性形态学变化密切相关。
Objective： To investigate the relation of morphological change and expression of nerve growth factor（NGF） and c - fos in submandibular gland from C57BL/ksj - db/db mutant diabetic mice. Method： The pure zygote, that is db/db mouse, displays obesity and high blood glucose over 10mmol/L. The experimental group used 3,4,6,8,10 month - old db/db mouse and the age - matched db/＋ m mouse as controis. Immunohistochemistry technology was applied in the study. Result： In db/db diabetic mice, submandibular gland atrophied and showed the significant proliferating interstitial fiber. The number of NGF in submandibular gland was respectively increased and decreased than that of age - matched control animals. The number of c - fos immunopositive ceils in submandibular gland was respectively increased and decreased than that of age - matched control animals. Conclusion： The lower level of NGF may be related to the development of diabetic neuropathy, db/db mutant diabetes results in of c -fos. The intestitial morphological changes occurred in submandibular gland. The change of c -fos expression may be relatived to atrophied parenchyma.
出处
《河北医学》
CAS
2006年第11期1081-1083,共3页
Hebei Medicine
