摘要
根据已报道的反转录转座子的序列设计合成一对引物,以西安绿茄基因组DNA为模板,采用PCR扩增的方法扩出一条DNA特异片段并克隆到pMD18-T载体中。采用一种新建立的在可见光下参照位置标记方法回收目的DNA片段,并与常规的在紫外光下回收的目的DNA片段进行了克隆效果比较。结果表明,在紫外光下回收的茄子反转录转座子基因片段易出现目的DNA片段断裂,而在可见光下参照位置标记回收DNA的方法能保证目的DNA片段的完整性。
PCR primers were designed based on the reported sequences of plant retrotransposon. Xi an green eggplant was used as experimental material. A DNA fragment was amplified by polymerase chain reaction and cloned into the vector pMD18-T. The aimed DNA fragment was reclaimed by using a new method which was based on marking position in visible light and a groovy method which was completed in ultraviolet. The cloning effect comparison of the two methods showed that the eggplant retrotransposon gene fragment was not integral using the groovy method and the new method could keep the aimed DNA fragment integrality.
出处
《北京农学院学报》
2006年第3期5-8,共4页
Journal of Beijing University of Agriculture
基金
北京市自然科学基金资助项目(项目编号:5982006)
关键词
茄子
反转录转座子
克隆
回收方法
eggplant
retrotransposon
cloning, reclaiming methods
