摘要
目的观察重型肝炎患者血清对人肝癌Bel-7402细胞生长抑制率的影响。方法重型肝炎患者血清与Bel-7402细胞共同培养,应用四氮唑蓝比色分析法(MTT法)检测细胞生长抑制率,并检测细胞培养上清液中ALT、LDH。结果1、Bel-7402细胞的生长抑制率随添加的重型肝炎患者血清浓度升高而增加。当血清浓度为50%、培养48小时细胞生长抑制率为73%,与对照组比较有显著差异。2、25%血清浓度作用下,各培养时间细胞培养上清液中ALT、LDH水平,实验组与对照组无显著性差异。结论重型肝炎患者血清中的物质对肝癌细胞的生长有明显的抑制作用。
Objective This study was to investigate the effect of serum substances in patients with chronic fulminant hepatitis complicated with HE （Ⅱ） on growth-suppressor rate of Bel-7402 cell , Methods The Bel- 7402 cell was cuhurod in vitro with serum of fulminant hepatitis. According to MTT, the OD value was detected and then cell suppressor ratio was obtained. The damage condition of cell membrane was deduced by detecting the releasing ratio of ALT and LDH in supematant. Results 1,The growing suppressor ratio of Bel-7402 cells increased with serum concentration in the test group through MTT examination, When the serum concentration is up to 50%, growth suppressor ratio is 73% after cultured 48 hours. There is significant difference between the test group and control group. 2. Checking releasing ratio of ALT and LDH in supematant of cell cultured with 25% serum at different time （0, 8, 24 or 48hrs）, there was no significant difference between test group and control group.
出处
《海南医学》
CAS
2007年第1期7-8,共2页
Hainan Medical Journal
