摘要
目的为探讨昆明山海棠总生物碱(alkaloids from Tripterygium Hypoglaucum Hutch,THHa)抗肺癌机制,研究THHa诱导肺腺癌A549细胞凋亡与细胞周期改变的关系。方法应用形态学观察、DNA凝胶电泳、流式细胞仪检测分析肺腺癌A549细胞凋亡及细胞周期改变。结果THHa能够诱导肺腺癌A549细胞凋亡并主要作用于细胞周期S期(S期细胞增多)。结论THHa可能通过诱导细胞凋亡而抑制A549细胞增殖,这种作用与细胞周期有关,这些有助于将来肺癌治疗方案的合理设计。
Objective To explore the mechanism of the alkaloids from tripterygium hypoglaucum hutch （THHa） on the apoptosis of lung cancer. Methods Apoptosis and cell cycle of human lung adenocarcinoma cell line A549 were detected by morphological observation, DNA electrophoresis, and flow cytometry. Results THHa could induce apoptosis of human lung adenocarcinoma cell line and arrest the cells at S phase of cell cycle. Conclusion Apoptosis contributes to inhibitory effect of THHa on A549 cells. This may be helpful in designing an appropriate chemotherapeutic regimen in future.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第1期18-20,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30100241)~~
关键词
昆明山海棠
肺腺癌A549细胞
细胞凋亡
细胞周期
tripterygium hypoglaucum hutch
lung adenocarcinoma A549 cell line
apoptosis
cell cycle
