摘要
目的探讨低氧对体外培养大鼠睾丸支持细胞的影响。方法应用大鼠睾丸支持细胞与生精细胞共培养系统,观察低氧条件下支持细胞的存活数,考马斯亮蓝染色后细胞骨架变化以及生精细胞脱落数。结果在低氧条件下,支持细胞存活率显著降低,支持细胞细胞骨架出现松弛、回缩等现象;脱落生精细胞数随低氧时间的延长而增加,有明显时间-效应关系。结论低氧可引起大鼠睾丸支持细胞形态结构及功能的损伤。
Objective To explore the effect of hypoxia on cultured rat Sertoli cells in vitro. Methods In a Sertoli-germ cells co-culture system, the effect of hypoxia on the morphological characteristics of Sertoli cells were observed, including the number of Sertoli cells, the cytoskeleton changes after Coomassie brilliant blue staining and the detachment of germ cells. Results The loosing and retracting of cytoskeleton in Sertoli cells was found. Both survival rate of Sertoli cells and detachment of germ cells showed time-response relation. Conclusion Hypoxia damages Sertoli cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第1期65-67,共3页
Journal of Third Military Medical University
