摘要
目的探讨癫痫持续状态(status epilepticus,SE)后海马细胞凋亡的发生及其与caspase-3表达的关系。方法采用匹罗卡品诱发大鼠SE模型,用TUNEL染色和免疫组化技术检测海马神经元凋亡和caspase-3表达的动态变化。结果正常对照组大鼠海马未见TUNEL阳性细胞;SE后6 h,开始出现少量TUNEL阳性细胞;SE后72 h,达到高峰;SE后7 d,TUNEL阳性细胞开始减少。正常对照组大鼠海马可见少量caspase-3阳性表达;SE后6 h,大鼠海马caspase-3阳性表达增多,主要集中于CA1和CA3区;SE后48 h,caspase-3表达达到高峰;SE后72 h^7 d,caspase-3阳性染色细胞数开始减少;但仍显著多于对照组,差异有极显著意义(P<0.01)。结果显示caspase-3表达分布与TUNEL染色基本一致,caspase-3表达高峰早于TUNEL所示凋亡细胞出现的高峰。结论神经元凋亡参与了SE后海马神经元迟发性死亡过程并与caspase-3的激活有密切的关系。
Objective To probe the occurrence of neuroal apoptosis in rats after status epilepticus （SE） and the relationship between neuron apoptosis and caspase-3 expression. Methods Rat model of SE was induced by intraperitoneal injection of 300 mg/kg pilocarpine in 20 male Wistar rats. Another 5 rats served as control. The apoptotic neuron was detected by TdT-mediated dUTP Nick End Labeling （TUNEL） , and caspase- 3 expression was observed by immunocytochemistry at 6^th , 48^th , 72^th h and 7^th d after SE induction. Results In control rats, TUNEL-positive neuron could not be found in hippocampus. The number of TUNEL-positive neuron began to increase at 6^th h after SE induction and peaked at 72^th h, began to decrease at 7^th d after SE induction. In control rats, there were few caspase-3 expression in hippocampus. The caspase-3 immunoreactivity began to increase at 6^th h after SE and peaked at 48^th h, especially in CA1 and CA3 region of hippocampus, began to decrease at 72^th h and dropped to normal level at 7^th d. The results indicated that the distribution of caspase-3 expression was consistent with the distribution of TUNEL-positive neuron. The peak of caspase-3 expression was earlier than that of TUNEL-positive neuron. Conclusion Neuron apoptosis participated in the process of delayed neuronal death in hippocampus after SE and was correlated with caspase-3 activation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第1期71-73,共3页
Journal of Third Military Medical University
