摘要
目的了解临床分离的耐亚胺培南铜绿假单胞菌(IRPa)中Ⅰ类整合子及β-内酰胺类抗生素耐药相关基因存在状况。方法自临床分离28株IRPa,采用聚合酶链反应及序列分析的方法检测Ⅰ类整合子遗传标记(intI1和qacE△1-sul1基因)及18种β-内酰胺类抗生素耐药相关基因。结果28株中,intI1、qacE△1-sul1、blaTEM、blaOXA-10群和blaCARB基因阳性株数(%)分别为21株(75.0%)、24株(85.7%)、20株(71.4%)、1株(3.6%)和1株(3.6%),经序列分析分别确认为blaOXA-10(GenBank注册号:AY841859)和blaCARB-3。25株(89.3%)oprD基因缺失,blaIMP、blaVIM、blaSHV、blaPER、blaVEB、blaGES、blaMIR、blaDHA、blaSPM、blaGIM、blaOXA-1群、blaOXA-2群、blaBEL-1、blaCTX-M-1群基因均阴性。结论临床分离的IRPa中Ⅰ类整合子携带率很高,至少存在blaTEM、blaOXA-10和blaCARB-33种β-内酰胺酶基因,oprD基因缺失突变是其对亚胺培南耐药的主要原因。
Objective To investigate the class 1 integron and the drug-resistant genes associated β-lactam antibiotics in imipenem-resistant Pseudomonas aeruginosa （IRPa） isolated from the clinical specimens. Methods The class 1 integron genetic markers （including intI1 and qacE Δ1-sull） and 18 kinds of the drug-resistant genes associated β-lactam antibiotics were analyzed from 28 isotated strains of IRPa by PCR and verificated by DNA sequencing. Results In 28 strains of IRPa, the positive rates of int11, qacE Δ1-sull, blaTEM, blaoxA-10 group, and blaCARB were 75.0%, 85.7%, 71.4%, 3.6%, and 3.6%, respectively, blaoxA-10 group and blaCARB were sequeneed and determined to be blaoxA-10 subtype ESBL gene （GenBank accession number： AY841859） and blaCARB-3, respectively. The deficiency rate of oprD gene was 89.3%. Genes of blarm, blaw, blaSHV, blaPER, blaVEB, blaCES, blaMIR, blaDHA, bl aSPM, blaGM, blaoxA-1 group, blaoxA-2 group, blaBEL-1, and blaCTX-M-1 group showed all negative. Conclusion There are very high harbouring rates of the class 1 integron in IRPa isolated clinically,and at least 3 kinds of β-lactamases gene existed including blab, blaoxA-10, blacCARB-3. The oprD gene lack mutation were the main mechanism of Pseudomonas aeruginosa resistant to imipenem.
出处
《江西医学检验》
2006年第6期491-494,共4页
Jiangxi Journal of Medical Laboratory Sciences
