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Cobe Spectra血细胞分离机采集干细胞的效果观察 被引量:1

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摘要 目的探讨CobeSpectra血细胞分离机MNC程序采集外周血干细胞的效果。方法用CobeSpectra血细胞分离机的MNC程序采集健康供者和肿瘤患者外周血干细胞;供者给予G-CSF动员,患者采用化疗加G-CSF动员,采集的干细胞用流式细胞仪检测CD34+抗原表达细胞数。供/患者10人次共采集15次,处理抗凝全血(11420±2401)ml,时间(236±28)min,抗凝剂(975±195)ml。结果采集CD34+细胞(218.14±243.53)x106,MNC的采集效率为(51.02±29.85)%,单采后供/患者Plt、HB、Hct分别下降15.38%、11.22%、11.37%;在整个采集过程中未观察到严重的副反应。结论CobeSpectra血细胞分离机的MNC程序能采集到血小板含量低,高产量有效的自体/异体CD34+细胞。
机构地区 浙江省血液中心
出处 《江西医学检验》 2006年第6期515-516,共2页 Jiangxi Journal of Medical Laboratory Sciences
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参考文献5

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